EFEK C-10 MASSOIALACTONE TERHADAP KULTUR MULTISPESIES BIOFILM

HASYRUL HAMZAH, Dr. Sylvia Utami Tunjung Pratiwi.,M.Si; Dr. rer. nat. Triana Hertiani,M.Si.,Apt

2017 | Tesis | S2 Ilmu Farmasi

C-10 masoaialactone merupakan komponen utama dari minyak atsiri kulit batang Massoia aromatica Becc. yang berpotensi sebagai antibakteri dan antifungi. Penelitian ini bertujuan untuk mengetahui efek pemberian C-10 Massoialactone terhadap kultur multispesies biofilm. C. albicans, P. aeruginosa, E. coli. Minyak masoyi diperoleh dari hasil destilasi uap dan air. Isolasi C-10 Massoialactone dilakukan dengan metode KLT. Analisis fitokimia berupa kromatografi lapis tipis (KLT) dan gas chromatography � mass spectrometry (GC-MS), penghambatan pembentukan fase pertengahan, pematangan dan degradasi multispesies biofilm melalui metode broth microdillution. diamati pembacaan optical density menggunakan microplate reader pada panjang gelombang 595 nm, transmission electron microscope (TEM) dan Scanning electron microscope (SEM) dilakukan untuk melihat perubahan ultrastructural multispesies biofilm . Hasil GC-MS mengandung senyawa C-10 Massoialactone sebesar 96.59%. C-10 Massoialactone memiliki aktivitas penghambatan pada fase pertengahan maupun pematangan dan degradasi biofilm, baik mono, dual maupun multispesies, dibuktikan dengan penurunan pertumbuhan biofilm seiring dengan kenaikan konsentrasi isolat. Nilai (MBIC50) C-10 Massoialactone dalam menghambat pembentukan fase pertengahan biofilm mono - spesies E. coli, P. aeruginosa dan C. albicans sebesar 0,231 % v/v; 0,225 % v/v dan 0,224 % v/v, fase pematangan sebesar 0,264 % v/v; 0,357 % v/v; 0,406 %, dual � spesies biofilm C. albicans � E. coli (0,402 % v/v; 0,290 % v/v), dan dual - spesies P. aeruginosa � C. albicans (0,446 % v/v; 0,167 % v/v) serta multispesies biofilm P. aeruginosa � C. albicans � E. coli (0,532 % v/v; 0,818 % v/v). Hasil analisa TEM pada biofilm mono-spesies C. albicans, P. aeruginosa, E. coli menunjukkan adanya lisis dan pemecahan sel. Hasil analisa SEM menujukkan terjadinya kerusakan sel dan kebocoran sel disebabkan sebagai penghambatan C-10 Massoialactone. �

C-10 masoaialactone is a major component of the essential oil of Massoia Aromatica Becc bark which has a potential as an antibacterial and antifungal. This study aims to determine the effect of C-10 Massoialactoneto the culture of multispecies biofilm of C.albicans, P. aeruginosa, E. coli. Essential oil (Massoyi oil) obtained from the distillated of steam and water. The isolation of C-10 Massoialactone was done by TLC method. Phytochemical analysis was performed using a Thin Layer of Chromatography (TLC) and gas chromatography - mass spectrometry (GC-MS). Microtiter brote dilution method was employed to asses antibiofilm formation and biofilm degradation of C-10 Massoialactone against dual and multispesies biofilm. Transmission electron microscope (TEM) and Scanning electron microscope (SEM) were used for visualization in analysis of biofilm structure. The GCMS result revealed the presence of C-10 Massoialactone (96.59 %). C-10 Massoialactone a dose defendant activity to inhibit intermediate & mature phase of as well as to degrade ferformed biofilms. The concentration of C-10 Massoialactone required to inhibit 50 % mono-spesies, intermediate phase of E. coli, P. aeruginosa, C. albicans biofilms. Was 0,231 % v/v, 0,225 % v/v, & 0,224 % v/v respectively, where as higher concentration of C-10 Massoialactone required to inhibit was mature phase of mono, dual & multispesies biofilm of the test organism. The result of TEM analysis in a mono-species biofilms of C. albicans, P. aeruginosa, E.coli showed that there were lysis and cell division. Whereas SEM analysis showed that there were cells damage and cells leakage caused by C-10 Massoialactone. �

Kata Kunci : C-10 Massoialactone, multispecies biofilm, C. albicans, P. aeruginosa, E. coli.