PENGARUH ASAM URAT TERHADAP EKSPRESI INTERCELULLAR ADHESION MOLECULES-1(ICAM-1) DAN INFILTRASI MAKROFAG PADA HEPAR MENCIT(Mus musculus)

ARDIKA ANANDA, Dr.dr. Dwi Cahyani Ratna Sari, M.Kes., PA(K); dr. Nur Arfian, Ph.D; dr. Nungki Anggorowati, Sp.PA, Ph.D

2017 | Skripsi | S1 PENDIDIKAN DOKTER

Latar Belakang: Hiperurisemia tiap tahun terus meningkat prevalensinya di dunia. Keadaan hiperurisemia dikaitkan dengan peningkatan mortalitas. Hiperurisemia dikaitkan dengan penyakit non alcoholic fatty liver disease dan dikaitkan dengan keparahan penyakit hepatitis C kronis. Tujuan: Tujuan dari penelitian ini adalah mengetahui pengaruh asam urat terhadap inflamasi di hepar yang komponennya diukur melalui ekspresi ICAM-1 dan infiltrasi makrofag. Metode: Mencit jantan Swiss background (usia 3-4 bulan, berat 30-50 g) dibagi menjadi 3 kelompok, yaitu (1) kelompok Kontrol; (2) kelompok asam urat 125mg/KgBB/hari selama 21 hari (AU21); dan (3) kelompok asam urat 125mg/KgBB/hari selama 28 hari (AU28). Masing-masing kelompok berisi 4-5 mencit. Setelah mencit diterminasi, pengambilan sampel hepar dilakukan untuk ekstrasi RNA dan blok paraffin. Ekspresi ICAM-1 dan CD68 dinilai menggunakan Reverse-Transcriptase PCR(RT-PCR). Jumlah makrofag dinilai dengan pengecatan IHC CD68. Hasil: Terdapat peningkatan bermakna ekspresi ICAM-1 dan jumlah makrofag pada kelompok AU21(p<0,05) dan AU28 (p<0,05) terhadap kelompok Kontrol. Peningkatan bermakna ekspresi ICAM-1 dan jumlah makrofag juga terdapat pada kelompok AU28(p<005) terhadap kelompok AU21. Terdapat peningkatan bermakna ekspresi CD68 pada kelompok AU28(p<0,05) terhadap kelompok Kontrol. Kesimpulan: Asam urat menyebabkan peningkatan ekspresi ICAM-1 dan infiltrasi makrofag pada hepar mencit.

Background: Wordwide, the prevalence of hyperuricemia has increased every year. Hyperuricemia has been associated with increased mortality. Hyperuricemia is associated with non-alcoholic fatty liver disease and associated with chronic hepatitis C disease severity. Aim: This study is aimed to determine the effect of uric acid on inflammation in the liver whose component measured by expression of ICAM-1 and macrophage infiltration. Method: Male Swiss background mice (aged 3-4 months, weighted 30-50 g) were divided into 3 groups, namely (1) Control group; (2) uric acid 125mg/KgBB/day for 21 days (AU21); (3) uric acid 125mg/KgBB/day for 28 days (AU28). Each group contained 4-5 mice. After the mice were terminated, liver sample were taken for RNA extraction and paraffin block. The expression of ICAM-1 and CD68 were assessed using Reverse-Transcriptase PCR(RT-PCR). Total macrophage was assessed by CD68 IHC staining. Results: There was a significant increase in the expression of ICAM-1 and total macrophage at AU21 group (p<0,05) and AU28 group (p<0,05) compared to the Control group. Significant increase in the expression of ICAM-1 was also seen on AU28 group (p<0,05) compared to the AU21 group. There was a significant increase in the expression of CD68 on AU28 group (p<0,05) compared to the AU21 group. Conclusion: Uric acid increases ICAM-1 expression and macrophage infiltration in mice liver.

Kata Kunci : Asam urat, ICAM-1, CD68, makrofag / Uric acid, ICAM-1, CD68, macrophage