Aktivitas Sitotoksik Self Nano Emulsifying Drug Delivery Systems (SNEDDS) Kurkumin dan SNEDDS Pentagamavunon-0 Terhadap Sel Kanker Payudara 4T1 dan Sel Normal Vero

DEBI OCTAVIA HARYONO, drh. Retno Murwanti, M.P., PhD.; Dr. rer. nat. Ronny Martien, M.Si.

2017 | Skripsi | S1 FARMASI

Senyawa kurkumin dan pentagamavunon-0 (PGV-0) merupakan kandidat agen kemoprevensi yang potensial, namun keduanya sukar larut dalam air sehingga bioavailibilitasnya di dalam tubuh rendah. Formulasi Self Nano Emulsifying Drug Delivery Systems (SNEDDS) telah dikembangkan untuk meningkatkan kelarutan dan bioavalibilitas senyawa tersebut. Penelitian ini dilakukan untuk menguji aktivitas sitotoksik SNEDDS kurkumin dan SNEDDS PGV-0 terhadap sel kanker payudara 4T1 dan sel normal Vero. Aktivitas sitotoksik SNEDDS kurkumin dan SNEDDS PGV-0 diuji dengan metode MTT assay. Sejumlah 5x103 sel 4T1 per sumuran dan 10x103 sel Vero per sumuran dalam 96-well plate diberi perlakuan SNEDDS kurkumin dan SNEDDS PGV-0 dengan berbagai seri konsentrasi. Setelah 24 jam, dilakukan penambahan reagen MTT dan stopper, dilanjutkan pembacaan absorbansi menggunakan microplate reader pada lamda 595 nm. Hasil uji dianalisis dengan metode regresi linear menggunakan software Microsoft Excel 2010 untuk mendapatkan nilai IC50. Hasil penelitian menunjukkan bahwa SNEDDS kurkumin dan SNEDDS PGV-0 memiliki aktivitas sitotoksik pada sel 4T1 dengan nilai IC50 masing-masing 5,48 plus minus 0,36 mikrogram per mililiter dan 5,36 plus minus 0,56 mikrogram per mililiter, sedangkan pada sel Vero didapatkan nilai IC50 masing-masing 4,40 plus minus 0,17 mikrogram per mililiter dan 7,50 plus minus 0,30 mikrogram per mililiter. Hasil uji signifikansi menggunakan paired T-test menunjukkan nilai IC50 SNEDDS kurkumin dan kurkumin maupun SNEDDS PGV-0 dan PGV-0 yang berbeda signifikan pada sel 4T1 dan sel Vero, mengindikasikan bahwa formulasi SNEDDS memberikan pengaruh terhadap aktivitas kurkumin dan PGV-0 pada sel 4T1 dan sel Vero.

ABSTRACT Curcumin and pentagamavunon-0 (PGV-0) are potential candidates of chemopreventive agents, however curcumin and PGV-0 are poorly soluble in water so their bioavailibilities inside the body are low. The Self Nano Emulsifying Drug Delivery Systems (SNEDDS) formulation has been developed to increase solubility and bioavailibilty of curcumin and PGV-0. The purpose of this study was to investigate cytotoxic activity of SNEDDS curcumin and SNEDDS PGV-0 against 4T1 breast cancer cell and normal cell Vero. Cytotoxic activity of SNEDDS curcumin and SNEDDS PGV-0 was tested using the MTT assay. 5x103 4T1 cells and 10x103 Vero cells per well in a 96-well plate were treated with SNEDDS curcumin dan SNEDDS PGV-0 in a various concentrations. After 24 hours, MTT dan stopper reagent were added, and the absorbance was read using microplate reader at lamda 595 nm. The test result was analyzed using Microsoft Excel 2010 to obtain the IC50 value. The result of this study indicated that SNEDDS curcumin dan SNEDDS PGV-0 had cytotoxic activity against 4T1 cell with IC50 values respectively 5,48 plus minus 0,36 microgram per mililiter and 5,36 plus 0,56 microgram per mililiter, whereas in Vero cell the IC50 values obtained are respectively 4,40 pus minus 0,17 microgram per mililiter and 7,50 plus minus 0,30 microgram per mililiter. Test of significance was done with paired T-test, showing that the comparison of IC50 from SNEDDS curcumin and curcumin and IC50 from SNEDDS PGV-0 and PGV-0 are significantly different in 4T1 cell and in Vero cell. This result indicated that SNEDDS formulation gave influence in curcumin and PGV-0 cytotoxic activity. Keywords: SNEEDS, Curcumin, PGV-0, 4T1.

Kata Kunci : SNEDDS,Kurkumin,PGV-0,4T1