EFEK REBUSAN DAUN SALAM (Syzygium polyanthum (Wight) Walp.) TERHADAP DENSITAS OPTIK BAKTERI PLAK

VITA WAHYU HIDAYANI , Dr. drg Juni Handajani, M.Kes, Ph.D; drg. Tetiana Haniastuti, M.Kes, Ph.D

2016 | Skripsi | S1 ILMU KEPERAWATAN GIGI

INTISARI Plak adalah lapisan tipis yang melekat pada permukaan gigi dan struktur keras lain di rongga mulut. Plak terdiri dari 70% bakteri, seperti Streptococcus mutans, Actinomyces naeslundii, Streptococcus sanguinis, Niesseria sp. dan lain-lain. Daun salam (Syzygium polianthum (Wight) Walp.) mengandung flavonoid, tanin, dan minyak atsiri yang memiliki aktifitas antibakteri. Penelitian ini bertujuan untuk mengetahui efek rebusan daun salam terhadap pertumbuhan bakteri plak. Sampel plak diambil dari permukaan bukal gigi molar pertama atas. Sampel kemudian diinokulasi pada media BHI dan diinkubasi selama 370C selama 24 jam. Rebusan daun salam konsentrasi 50% (uji) dan akuades (kontrol) ditambahkan kedalam suspensi bakteri 1,5x108 CFU/ml. Tabung reaksi diinkubasi selama 24 jam pada suhu 370C. Pengukuran densitas optik menggunakan spektrofotometer pada panjang gelombang 600 nm. Analisis data menggunakan t-test. Hasil menunjukkan densitas optik kelompok uji lebih rendah dari kelompok kontrol. T-test menunjukkan perbedaan bermakna densitas optik antar kelompok (p<0,05). Kesimpulan penelitian ini yaitu rebusan daun salam efektif dalam menurunkan pertumbuhan bakteri plak ditandai penurunan densitas optik.

Plaque is a thin layer attached to the tooth surface and other hard structures in the oral cavity. It consists of 70% bacteria such as Streptococcus mutans, Actinomyces naeslundii, Streptococcus sanguinis, Niesseria sp., etc. Bay leaf (Syzygium polyanthum (Wight) Walp.) contains flavonoids, tannins, and essential oils which has antibacterial activity. This study aimed to determine the effect of bay leaf decoction on growth of plaque bacteria. Plaque samples was taken from the buccal surface of upper first molar. The sample was then inoculated in BHI media and incubated at 37oC for 24 hours. Fifty percent bay leaf decoction (treatment) and distilled water (control) was added into 1.5x108 CFU/ml bacterial suspension. The tube was incubated for 24 hours at 370C. The optical density was measured using a spectrophotometer at 600 nm wavelength. Data were analyzed using T-test. Result showed optical density of the treatment group was lower than the control group. T-test showed significant differences of the optical density between the groups (p<0,05). In conclusion, 50% bay leaf decoction reduces bacterial plaque growth which characterized by a decrease in the optical density.

Kata Kunci : Bakteri plak, rebusan daun salam, densitas optik.