PROFIL BIOKIMIA DARAH SAPI PERANAKAN FRIESIAN HOLSTEIN YANG KAWIN BERULANG DI UNIT PELAKSANA TEKNIS TERNAK PERAH FAKULTAS PETERNAKAN UNIVERSITAS GADJAH MADA

NOVI MAULIDA, Ir. Diah Tri Widayati, MP., Ph.D. ; Dr. Ir. Adiarto. M.Sc.

2017 | Skripsi | S1 ILMU DAN INDUSTRI PETERNAKAN

Penelitian ini bertujuan untuk mengetahui profil biokimia darah yang terdiri dari glukosa darah, kolesterol kadar, dan urea darah pada sapi perah Peranakan Friesian Holstein (PFH) yang mengalami kejadian kawin berulang di Unit Pelaksana Teknis (UPT) Ternak Perah Fakultas Peternakan Universitas Gadjah Mada (UGM). Tahap awal penelitian adalah identifikasi sapi normal dan yang mengalami kawin berulang melalui data recording. Identifikasi siklus estrus dilakukan setiap hari selama dua kali siklus estrus untuk mengetahui siklus estrus ternak. Sampel darah diambil seminggu sekali sebelum diberi pakan melalui vena caudalis menggunakan vaccum holder dengan tabung EDTA. Pengambilan sampel darah dilakukan sebanyak tiga kali. Analisis sampel darah dilakukan di Laboratorium Penelitian dan Pengembangan Terpadu UGM. Data yang diperoleh dianalisis dengan menggunakan analisis independent sample t-test untuk mengetahui adanya perbedaan profil biokimia darah pada sapi perah yang kawin berulang dengan sapi perah normal. Hasil Kadar glukosa, kolesterol dan urea darah pada sapi perah yang mengalami kawin berulang adalah 58,53 mg/dl ; 154,81 mg/dl dan 28,10 mg/dl sedangkan kadar glukosa, kolesterol dan urea darah pada sapi perah yang normal adalah 61,47 mg/dl ; 196,06 mg/dl dan 31,99 mg/dl. Berdasarkan hasil penelitian dapat disimpulkan bahwa sapi PFH yang mengalami kawin berulang dengan sapi PFH normal kadar glukosa darah dan kolesterol darah tidak berbeda nyata, sedangkan kadar urea darah berbeda nyata.

The study was carried out to determine the profile of plasma glucose, plasma cholesterol and blood ureum nitrogen (BUN) of repeated breeding Friesian Holstein (FH) grade cows in the Dairy Processing Unit, Faculty of Animal Science, Gadjah Mada University. Six of FH grade cows were equally distribution into two groups i.e. normal breeding and repeated breeding FH grade cows. The Vaginal smear identification was done every days during two estrous cycles to determine the estrous stage. The blood samples were collected through vena caudalis used a vaccum holder with EDTA tube. The samples were collected three times every once a week. The blood samples were analysis for plasma glucose, plasma cholesterol and BUN in the Research and Development Integrated Laboratory. Then, the data analyzed using T-test. The result showed that there was no significant difference of plasma glucose and plasma cholesterol between the repeat breeding and normal breeding cows. The averages of plasma glucose and plasma cholesterol of repeated breeding cows were 58.53 mg/dl and 154.81 mg/dl whereas of normal breeding cows were 61.47 mg/dl and 196.06 mg/dl. Futhermore, there was a signficant difference of BUN between the repeated breeding and normal cows. The averages BUN of repeated breeding and normal breeding FH cows were 28.10 mg/dl dan 31.99 mg/dl. It can be concluded that repeated breeding FH cows have non significant difference plasma glucose and plasma cholesterol concentrations to the normal breeding FH cows, meanwhile the BUN concentration is significant difference.

Kata Kunci : glukosa darah, kawin berulang sapi PFH, kolesterol darah, dan urea darah