PERBANDINGAN EFEK ANTIOKSIDAN VITAMIN C DENGAN VITAMIN E PADA MODEL KONTUSIO PULMONER

WAWAN SUCI NURASTI, DR. dr. Supomo, Sp.B., Sp.BTKV

2016 | Tesis-Spesialis | SP ILMU BEDAH

Latar Belakang: Kontusio pulmoner merupakan trauma intratorakal yang berhubungan dengan trauma tumpul pada dada yang paling sering didiagnosis dan terdapat pada 17-25% pasien trauma tumpul dada. Kontusio pulmoner juga merupakan faktor risiko independen terjadinya pneumonia, acute lung injury (ALI)klinis akut, dart acute respiratory distress syndrome (ARDS). Tujuan: Untuk membandingkan efek pemberian vitamin C dengan vitamin E pada model kontusio pulmoner Metode: 30 mencit dibagi menjadi 3 kelompok secara acak.Kelompok I dilakukan kontusio pulmo tanpa diberi vitamin C dan vitamin E, sedang kelompok II mendapatkan vitamin C dosis pemberian 100 mg/kgBB melalui intramuskuler, kelompok III mendapatkan vitamin E dosis pemberian 120 IU melalui intramuskuler. Semua sampel dilakukan analisis gas darah dan pemeriksaan histopatologi organ pulmo. Hasil: Pada kelompok vitamin C dari hasil analisis gas darah, rerata pH 7,1181+0,040, pO242,34+15,23, pCO284,94+9,73, cHCO326,78+1,45. Pada kelompok vitamin E dari hasil analisis gas darah, rerata pH 7,17+0,03, pO237,8+3,94, pCO262,55+6,66, cHCO322,74+1,60. Didapatkan perbedaan bermakna pada pH, pCO2 dan cHCO3 dengan p-value < 0,05. Pada kelompok vitamin C dari skoring patologi anatomi, rerata kongesti 0,60+0,84, oedema 0,50+0,707, hemoragi 0,30+0,48, radang 1,30+0,48. Pada kelompok vitamin E dari skoring patologi anatomi, rerata kongesti 1,00+0,81, oedema 0,90+1,10, hemoragi 0,70+1,05, radang 1,50+0,70. Tidak didapatkan perbedaan bermakna pada skoring patologi anatomi antara kelompok vitamin C dengan vitamin E dengan p-value > 0,05. Kesimpulan: Terdapat perbedaan bermakna pada pH, pCO2 dan cHCO3 dengan p-value < 0,05 pada kelompok vitamin C dibandingkan dengan kelompok vitamin E. Tidak didapatkan perbedaan bermakna pada skoring patologi anatomi antara kelompok vitamin C dengan vitamin E dengan p-value > 0,05. Kata kunci: Kontusio pulmoner, vitamin C, vitamin E.

Background : Pulmonary contusions is one of intrathoracic trauma associated with blunt trauma to the chest that is most often diagnosed and is present in 17-25 % of patients with blunt chest trauma . Pulmonary contusions can be an independent risk factor for the occurrence of pneumonia , acute lung injury ( ALI ), and acute respiratory distress syndrome ( ARDS ) . Purpose : The aim of theses research are comparing the effect of antioxidant vitamin C and vitamin E in pulmonary contusion model. Method : Thirty mice were divided into 3 groups randomly . Group I get pulmonary contusions but did not get any treatment, were the group II get vitamin C dosage of 100 mg / kg weight intramuscular , group III get vitamin E dosage of 120 IU by intramuscular administration . Examination of blood gas analysis and histopatology anatomy performed to evaluate the effect of treatment. Independent t test was used to test whether there are differences in the mean in each group. Result : Based on blood gas analysis in the group of vitamin C, the average pH of 7,1181+0,040, pO2 42,34+15,23, pCO2 84,94+9,73, cHCO3 26,78+1,45. In the group of vitamin E on the results of blood gas analysis, the average pH of 7,17+0,03, pO2 37,8+3,94, pCO2 62,55+6,66, cHCO3 22,74+1,60. Found significant differences in pH , pCO2 and cHCO3 with p - value < 0.05. In the group of vitamin C from scoring anatomical pathology, the average congestion of 0.60 + 0.84 , edema 0.50 + 0.707, hemorrhage 0.30 + 0.48, inflammation 1.30 + 0.48. In the group of vitamin E from scoring anatomical pathology, the average congestion of 1.00 + 0.81, edema 0.90 + 1.10, hemorrhage 0.70 + 1.05, inflammation 1.50 + 0.70. There were no significant differences in anatomical pathology scoring between the vitamin C and vitamin E with p - value> 0.05 . Summary : There were significant differences in pH, pCO2 and cHCO3 with p - value < 0.05 in the vitamin C group compared with the group of vitamin E. There were no significant differences in anatomical pathology scoring between the vitamin C group compared vitamin E group with p - value> 0.05. Key words: Pulmonary contusion, vitamin C, vitamin E.

Kata Kunci : Kontusio pulmoner,vitamin C,vitamin E