PENGARUH SUHU PENYIMPANAN BUBUK Sargassum hystrix TERHADAP AKTIVITAS ANTIOKSIDANNYA

PINGKAN MAYESTIKA AFGATIANI, Dr. Amir Husni, S.Pi., M.P ; Dr. Siti Ari Budhiyanti, S.T.P., M.P

2016 | Skripsi | S1 TEKNOLOGI HASIL PERIKANAN

Penelitian dilakukan untuk mengetahui pengaruh suhu penyimpanan terhadap aktivitas antioksidan pada bubuk Sargassum hystrix. Sampel berasal dari Pantai Sepanjang Gunung Kidul, Yogyakarta. Sampel yang telah dikeringkan dan dibubukkan diberikan perlakuan penyimpanan dengan suhu kamar (17-33°C), pendinginan (4°C), dan pembekuan (-32,8°C) selama 2 bulan dan diuji kadar air, total fenol, DPPH, dan FIC setiap 2 minggu sekali, serta pengujian GC-MS dilakukan sebelum disimpan dan setelah disimpan selama 2 bulan pada setiap perlakuan suhu penyimpanan. Kadar air pada ketiga perlakuan semakin meningkat seiring dengan lama penyimpanan yaitu berkisar 8,55%-14,27%. Kadar total fenol sebelum disimpan sebesar 175,73 mg GAE/g ekstrak. Suhu pembekuan memiliki kadar total fenol tertinggi setelah disimpan selama 2 bulan (22,07 mg GAE/g ekstrak). Pengujian DPPH menunjukkan bahwa S. hystrix memiliki nilai IC50 sebesar 0,45 mg/ml. Suhu pendinginan memiliki nilai IC50 RSA DPPH terendah yaitu sebesar 3,18 mg/ml setelah disimpan selama 2 bulan. Pengujian FIC menunjukkan bahwa S. hystrix memiliki nilai IC50 sebesar 1,44 mg/ml. Suhu pembekuan memiliki nilai IC50 FIC terendah yaitu sebesar 15,36 mg/ml. Analisis GC-MS pada sampel sebelum dilakukan penyimpanan terdeteksi 8 senyawa yang memiliki aktivitas antioksidan (86.96%). Setelah disimpan selama 2 bulan, senyawa yang terdeteksi memiliki aktivitas antioksidan pada suhu kamar sebanyak 4 senyawa (26.26%), pendinginan sebanyak 3 senyawa (35.98%), dan pembekuan sebanyak 8 senyawa (89.6%).

The study was conducted to determine the effect of storage temperature on the antioxidant activity in Sargassum hystrix. Samples collected from Sepanjang Beach Gunung Kidul, Yogyakarta. The samples were dried and pulverized given treatment storage with room temperature (17-33°C), refrigeration (4°C) and freezing (-32,8°C) for 2 months and tested the water content, total phenol, DPPH, and FIC every 2 weeks , as well as GC-MS analysis performed before storage and after storage for 2 months at a storage temperature of each treatment. The water content in the three treatments has increased along with storage time which ranges from 8.55% -14.27%. Levels of total phenols before storage is 175.73 mg GAE/g extract. Freezing temperatures have the highest levels of total phenols after being stored for 2 months (22.07 mg GAE/g extract). DPPH testing showed that Sargassum hystrix has IC50 value of 0.45 mg/ml. Cooling temperature has lowest IC50 value of DPPH RSA 3.18 mg/ml after stored for two months. Tests showed that Sargassum hystrix has IC50 value of FIC 1.44 mg/ml. The freezing temperature has the lowest FIC IC50 value 15.36 mg/ml. GC-MS analysis of the samples prior to storage detected 8 compounds that have antioxidant activity (86.96%). After being stored for two months, compounds that have antioxidant activity detected at room temperature as much as 4 compounds (26.26%), cooling by 3 compound (35.98%), and freezing as many as 8 compounds (89.6%).

Kata Kunci : antioksidan, penyimpanan, Sargassum hystrix, suhu, DPPH, FIC