CORRELATION BETWEEEN HEPARANASE AND GLOMERULOSCLEROSIS IN HYPERURICEMIA MODEL IN MICE

ODITRIO IRAWAN, dr. Nur Arfian, Ph.D.; dr. CH. Tri Nuryana, M Kes

2016 | Skripsi | S1 PENDIDIKAN DOKTER

LATAR BELAKANG: Penyakit ginjal kronis telah menjadi nmasalah global dalam bidang kesehatan masyarakat dikarenakan jumlah kasus yang terus meningkat, biaya yang tinggi serta hasil akhir yang buruk. heparanase diasosiasikan dengan degenerasi heparan sulfat di proteinuria, yang merupakan manifestasi dari glomerulosclerosis. TUJUAN: untuk menjelaskan korelasi antara heparanase dan glomerulosclerosis. METODE: Kami menginduksi tikus jenis swiss background ke dalam kondisi hiperurisemia dengan menginjeksikan asam urat. asam urat 125mg/kg/day dilarutkan dalam 0.2M NAOH dan diinjeksikan intraperitoneal dengan jumlah volume 0.2 ml selama 2 minggu. pengamatan dibagi menjadi 2 grup, ua7 dan ua14. skor glmerulosclerosis dinilai berdasarkan pengecatan PAS( periodic acid schiff). Expresi heparanase diukur menggunakan RT-PCR HASIL:Hiperurisemia meningkatkan skor glonmerulosclerosis di ua7 dan ua14 diikuti npeningkatan expresi heparanase di ua7 dan KESIMPULAN: Terdapat korelasi poisitif yang moderat antara heparanase dan glomerulosclerosis di model hiperurisemia pada mencit. KATA KUNCI: penyakit ginjal kronik, glomerulosclerosis, heparanase, hiperurisemia, mencit

BACKGROUND: CKD has become a burden for public health worldwide because of increment cases number, high costs, poor outcomes. Glomerulosclerosis is one of the marker for CKD. Heparanase has been associated in heparan sulfate degeneration in proteinuria which is one of the manifestation of glomerulosclerosis. PURPOSE: To elucidate the correlation between heparanase and glomerulosclerosis. METHOD: We induced male swiss background mice to hyperuricemia state by injecting uric acid. The uric acid 125mg/kg/day were dissolved in 0.2 M NAOH and was injected intraperitoneally with total liquid of volume 0.2mL until two weeks. The observation was divided into two group, UA7 and UA14. Glomerulosclerosis score was measured based on Periodic Acid Schiff (PAS) staining. Heparanase expression was measured using RT-PCR. RESULT:Hyperuricemia induced an increase of glomerulosclerosis score in UA7 and UA14, followed by increase of Heparanase expression in UA7 and UA14. CONCLUSION: There is a positive moderate correlation between heparanase and glomeruloslerosis in hyperuricemia model in mice. KEYWORDS: chronic kidney disease, glomerulosclerosis, heparanase, hyperuricemia, mice

Kata Kunci : penyakit ginjal kronik, glomerulosclerosis, heparanase, hyperuricemia, mencit