UJI DAYA ANALGETIK SENYAWA GARAM KALIUM DARI 4-(4'-HIDROKSI-3'-METOKSIFENIL)-3-BUTEN-2-ON PADA MENCIT JANTAN GALUR BALB/C DENGAN METODE GELIAT (WRITHING TEST)

HARDIYANTININGSIH, Dr. Nunung Yuniarti, S. F., Apt., M. Si.

2016 | Skripsi | S1 FARMASI

Gamavuton-0 dan mono-gamavuton-0 sudah terbukti memiliki efek analgetik. Kaliummonogamavuton merupakan garam kalium dari mono-gamavuton-0 (KMGVT-0). Namun senyawa ini belum pernah diteliti efek farmakologinya sebagai analgetik. Penelitian ini bertujuan untuk menguji efek analgetik pada mencit jantan galur BALB/C dengan metode geliat (Writhing Test) serta membandingkan parameter dengan potensi nilai ED50 senyawa KMGVT-0 dengan parasetamol. Sebanyak 70 mencit jantan galur BALB/C, dibagi dalam 10 kelompok. 5 kelompok diberi sediaan uji KMGVT-0 dosis 10, 20, 40, 80, dan 160 mg/kgBB per oral, 4 kelompok diberi parasetamol sebagai control positif dengan dosis 25, 50, 100 dan 200 mg/kgBB per oral, dan 1 kelompok diberi CMC Na 0,5% sebagai kontrol negatif dengan dosis 100 mg/kg BB, kemudian mencit diinduksi asam asetat 0,4% b/v dosis 100 mg/kgBB secara intraperitonial Pengamatan geliat setiap 5 menit selama 60 menit. Hasil penelitian menunjukkan bahwa daya analgetik pada KMGVT-0 lebih poten dibandingkan parasetamol di mana ED50 KMGVT-0 yaitu 36,50 mg/kg BB lebih kecil dibanding parasetamol yaitu 70,46 mg/kg BB.

Gamavuton-0 dan mono-gamavuton-0 has been proved to have analgesic effect. Kaliummonogamavuton is salt form of potassium from mono-gamavuton-0 (KMGVT-0). However, its analgesic effect has not been studied. The aim of this research is to test the analgesic effect of KMGVT-0 in male mice BALB / C strain using stretching method (writhing test) and to compare its analgesic potency with analgesic potency of paracetamol using ED50 as parameter. Total of 70 male mice BALB/C strain were divided into 10 groups, 5 groups were treated with KMGVT-0 orally (dose 10, 20, 40, 80, and 160 mg/kg BW), 4 groups were treated orally with paracetamol as positive control (dose 25, 50, 100 and 200 mg/kg BW), and 1 group for negative control treated with vehicle (0,5% Sodium CMC). Pain in mice was induced by i.p. injection of 0.4% acetic acid (dose 100 mg/kg BW). The writhing reflect was observed every 5 minutes for 60 minutes. The result of this research indicate that the analgesic potency of KMGVT-0 is more potent than paracetamol which is ED50 of KMGVT-0 is 36.50 mg/kg BW less than paracetamol 70.46 mg/kg BW.

Kata Kunci : kurkumin, kaliummonogamavuton, analgetik, Writhing Test