Pengaruh Konsentrasi Suspensi Cu-Zeolit Alam Aktif dalam Akuades terhadap Daya Antimikroba pada Candida albicans

NABILAH AULIA PUTRI, Dr. drg. Dyah Irnawati, MS. ; drg. Purwanto Agustiono, SU.

2016 | Skripsi | S1 PENDIDIKAN DOKTER GIGI

Denture stomatitis karena Candida albicans dapat diatasi dengan membersihkan gigi tiruan menggunakan agen pembersih kimiawi. Cu-zeolit alam aktif adalah material antimikroba gabungan logam berat tembaga dan material pembawa zeolit alam teraktivasi. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi suspensi Cu-zeolit alam aktif dalam akuades terhadap daya antimikroba pada C. albicans. Penelitian ini menggunakan zeolit alam (Wonosari), larutan HCl 2 M, serbuk CuCl2.2H2O (Merck, Jerman), biakan C. albicans, dan agar Sabouraud (Merck, Jerman). Zeolit alam (50 g) diaktivasi menggunakan 500 mL larutan HCl 2 M (1 jam/100oC), disaring, dicuci, dan dikeringkan (105oC/24 jam). Zeolit alam aktif lalu direaksikan dengan 250 mL larutan CuCl2 0,2 M (1 jam/100oC), disaring, dicuci, dan dikeringkan (100oC/24 jam). Serbuk Cu-zeolit alam aktif dicampurkan dengan akuades steril membentuk suspensi berkonsentrasi 9 mg/mL, 18 mg/mL, dan 36 mg/mL (n=4). Suspensi tersebut dikontakkan dengan C. albicans selama 120 menit, diencerkan, dan ditanam pada media agar sabouraud lalu diinkubasi (48 jam/37oC). Jumlah koloni yang tumbuh dihitung dalam satuan CFU/mL. Data dianalisis dengan uji Anava satu jalur (α = 0,05) dan LSD0,05. Rerata dan simpangan baku koloni setelah dikontakkan dengan suspensi berturut-turut adalah 36.800 ± 6.453 (konsentrasi 9 mg/mL), 31.675 ± 6.445 (konsentrasi 18 mg/mL), dan 22.375 ± 3.025 (konsentrasi 36 mg/mL) (CFU/mL). Hasil uji Anava satu jalur menunjukkan adanya pengaruh konsentrasi suspensi terhadap daya antimikroba pada C. albicans (p<0,05). Hasil uji LSD0,05 menunjukkan perbedaan bermakna antara kelompok konsentrasi suspensi 36 mg/mL dengan 9 mg/mL dan 18 mg/mL (p<0,05). Kesimpulan penelitian adalah konsentrasi suspensi Cu-zeolit alam aktif yang tinggi dapat meningkatkan daya antimikroba pada C. albicans.

Denture stomatitis caused by Candida albicans can be handled by cleaning the denture using chemical cleaning agent. Activated Cu-natural zeolite is an antimicrobial agent combination of copper and carrier agent activated natural zeolite. The aim of this study was to determine the effect of concentration of activated Cu-natural zeolite suspension in aquadest on antimicrobial activity in C albicans. Materials used were natural zeolite (Wonosari), 2 M HCl solution, CuCl2.2H2O powder (Merck, Germany), cultured C. albicans, and Sabouraud agar (Merck, Germany). Natural zeolite (50 g) was activated with 500 mL 2 M HCl solution (1 hour/100oC), filtered, washed, and dried (105oC/24 hours). Activated natural zeolite was reacted with 250 mL 0.2 M CuCl2 solution (1 hour/100oC), filtered, washed, and dried (100oC/24 hours). Activated Cu-natural zeolite was mixed with sterile aquadest thus forming suspension in 9 mg/mL, 18 mg/mL, and 36 mg/mL concentration (n=4). The suspension then was contacted with C. albicans for 120 minutes, diluted, and grown on Sabouraud agar, then incubated (48 hours/37oC). The number of colonies was counted in CFU/mL. Data were analyzed with one way ANOVA test (α = 0,05) and LSD0,05. The mean and standard deviation of the number of colonies after contacted with the suspension were 36.800 ± 6.453 (concentration of 9 mg/mL), 31.675 ± 6.445 (concentration of 18 mg/mL), and 22.375 ± 3.025 (concentration of 36 mg/ml) (CFU/mL), respectively. The result of one way ANOVA test showed that there is influence of the concentration of the suspension to antimicrobial activity in C. albicans (p<0,05). The result of LSD0,05 test showed significant differences between the concentration groups of the suspension 36 mg/mL with 9 mg/mL and 18 mg/mL (p<0,05). The conclusion was high concentration of activated Cu-natural zeolite could increase its antimicrobial activity against C. albicans.

Kata Kunci : Cu-zeolit alam aktif, Konsentrasi, Candida albicans, Antimikroba