Latar Belakang: Aplikasi fissure sealant merupakan salah satu upaya untuk mencegah terjadinya karies pada pit dan fisur gigi molar permanen. Aplikasi fissure sealant yang dilakukan di klinik JKG Poltekkes Kemenkes Yogyakarta belum pernah dilakukan evaluasi. Penelitian ini bertujuan untuk mengetahui faktor-faktor yang mempengaruhi keberhasilan aplikasi fissure sealant di klinik JKG Poltekkes Kemenkes Yogyakarta. Metode penelitian: Penelitian ini merupakan penelitian studi kasus, pendekatan kuantitatif non eksperimen. Subyek penelitian merupakan total populasi berjumlah 38 mahasiswa semester V JKG Poltekkes Kemenkes Yogyakarta 2011/2012. Alat ukur yang digunakan adalah kuesioner untuk mengukur pengetahuan, peran guru/pembimbing klinik, peran sarana dan prasarana, sikap, motivasi dan check list untuk mengukur keterampilan terhadap keberhasilan aplikasi fissure sealant di klinik JKG Poltekkes Kemenkes Yogyakarta. Analisis data menggunakan uji korelasi dan regresi logistik. Hasil Penelitian: Berdasarkan hasil analisis keberhasilan fissure sealant adalah baik 68,4% dan rusak 31,6%. Faktor yang mempunyai pengaruh bermakna terhadap keberhasilan aplikasi fissure sealant di klinik JKG Poltekkes Kemenkes Yogyakarta yaitu pengetahuan (p=0,001), keterampilan (p=0,001), peran guru/pembimbing klinik (p=0,020), sikap (p=0,005) dan motivasi (p=0,005). Faktor yang tidak mempunyai pengaruh bermakna terhadap keberhasilan aplikasi fissure sealant di klinik JKG Poltekkes Kemenkes Yogyakarta yaitu sarana dan prasarana (p=0,151). Kesimpulan: 1) Sarana dan prasarana tidak menunjukkan adanya pengaruh yang signifikan terhadap keberhasilan aplikasi fissure sealant di klinik JKG Poltekkes Kemenkes Yogyakarta. 2) Peningkatan pengetahuan, keterampilan, peran guru/pembimbing klinik, sikap dan motivasi akan meningkatkan keberhasilan aplikasi fissure sealant di klinik JKG Poltekkes Kemenkes Yogyakarta. 3) Variabel keterampilan memberikan kontribusi pengaruh terbesar yaitu sebesar 21,131% terhadap keberhasilan aplikasi fissure sealant di klinik JKG Poltekkes Yogyakarta.

Background: The application of fissure sealants is one of the efforts to prevent the incidence of caries in the pit and fissure in the permanent molar teeth. The application of fissure sealants at the Dental Health Department Clinics of Yogyakarta Health Polytechnic has never been evaluated. This research aims to identify the factors that contribute to the successfull application of fissure sealants at the Dental Health Department Clinic of Yogyakarta Health Polytechnic. Research Methods: This is a case study employing a non-experimental quantitative approach. Subjects were 38 fifth-semester students in Dental Health Departement in the 2011/2012 academic year. The measuring instrument used was a questionnaire survey to measure knowledge, the role of the clinical supervisor, the role of facilities and infrastructure, attitudes, motivation as well as a checklist to measure skills that contribute to the application of fissure sealants at the Dental Health Department Clinics. For purposes data analysis, logistic and multiple regression tests were used. Research Results: Analysis of the degrees of success in the application of fissure sealant showed the following results: 68,4% satisfactory, 31,6% broken. The factors that significantly contributed to the successful application of fissure sealants were knowledge (p=0,001), skills (p=0,001), the role of the clinical supervisor (p=0,020), attitudes (p=0,005), and motivation (p=0,005). The factors that had no significant contribution to the successful application of fissure sealants were the subjects’ of facilities and infrastructure (p=0,151). Conclusions: 1) The subjects’ of facilities and infrastructure do not indicate a significant influence on the successful application of fissure sealants at the Dental Health Department Clinics. 2) Increased knowledge and skills, the role of the clinical supervisor, attitudes and motivation enhance the quality of the application of fissure sealants. 3) The skills variable makes the greatest contribution, i.e. 21,131% to the successful application of fissure sealants at the Dental Health Department Clinic of Yogyakarta Health Polytechnic.

Kata Kunci : Pengetahuan, keterampilan, guru/pembimbing, sarana dan prasarana, sikap, motivasi.