Tujuan penelitian mengetahui efektivitas bawang putih (EBP) dalam pakan untuk detoksifikasi aflatoksin B1 (AFB1) pada ayam broiler. Penelitian dilakukan dua tahap: tahap pertama menguji efektivitas EBP terhadap AFB1 dengan uji per tumbuhan bakteri Bncillus nregn terium dan Stnphyllococcus epidermidis, sedangkan tahap kedua pengujian pada ayam broiler. Tahap pertama menggunakan rancangan acak kelompok. Perlakuan adalah P1: EBP, P2: residu hasil fraksinasi dengan pelarut heksan, P3: residu hasil fraksinasi dengan pelarut kloroform dan P4: residu hasil fraksinasi dengan pelarut metanol, sedangkan kelompok adalah konsentrasi senyawa hasil fraksinasi, sebagai berikut: Kl: 0 mg/ ml; K2: :2,5 mg/ rnl, K3: 5 mg/ml dan K4: 10 mg/ ml. Hasil tertinggi jumlah koloni bakteri yang hldup setelah dikontaminasi AFB1 diperoleh pada residu basil fraksinasi dengan pelarut metanol pada konsentrasi 2,5 mg/ ml. Tahap kedua menggunakan 144 ekor ayam broiler umur satu hari selama 42 hari. Ayam broiler dibagi 16 perlakuan, setiap perlakuan terdiri a tas sembilan ekor. Analisis data menggunakan rancangan acak lengkap pola faktorial, faktor A kadar AFB1 dan faktor B kadar EBP. Faktor A adalah: A1: 0 ppb (kontrol); A2: 500 ppb; A3: 1000 ppb; dan A4: 1500 ppb, faktor B: B1: 0% (kontrol); B2: 2%; B3: 4%; dan B4: 6%. Aflatoksin B1 diberikan per ornl dan EBP dicampurkan dalarn pakan. Hasil analisis d ata yang signifikan (P<0,05) dilakukan uji beda rata-rata menggunakan Duncan's New Multiple Range Test. Parameter untuk mengetahui efektivitas detoksifikasi adalah: konsurnsi pakan, pertambahan bobot badan (PBB), konversi pakan, bobot karkas, kadar kolesterol, kadar low density lipoprotei11 (LDL), kadar lrigh density lipoprotein (HDL), persentase lemak abdominal, bobot hati, bobot bursa fabrisius, kadar serum glutamin piruvat transaminase (SGPT) dan residu AFB1. Pengambilan data dilakukan dua kali, pada umur 21 dan 42 hari. Hasil analisis data menunjukkan perlakuan AFB1 mulai 500 ppb meningkatkan secara signifikan): konversi pakan, bobot hati, kadar SGPT, persentase lemak abdominal (21 hari), dan menurunkan HDL serta bobot bursa fabrisius (42 hari). Pacta kadar AFB1 1500 ppb menurunkan; PBB, bobot bursa fabrisius (21 hari) dan bobot karkas (42 hari), serta rneningkatkan kolesterol tota l dan LDL. Penggunaan EBP 2% menurunkan: LDL dan pe rsentase lemak abdominal (42 hari), meningkatkan HDL (21 hari), EBP 4% rnenurunkan persentase lemak abdominal (21 hari), sedangkan EBP 6% menurunkan: bobot hati dan kolesterol total (21 hari). Residu AFB1 paling tinggi pada hati perlakuan AFB11000 ppb, sedangkan daging pada AFB1 1500 ppb.

The purpose of the study was to evaluate the effect of garlic extract (GE) on rations to detoxification of aflatoxin Bt (AFBt). The study was conducted in two phases: the first phase was testing the effectiveness of GE to detoxification of AFBl by the inhibition of bacterial growth in Bncilltts megnteriwu and Stnplzyllococc/ls epidermidis, whereas the second phase was ill 7'i7'0 in broiler. Treatments were P1: GE; P2: hexane solvent fractionation; P3: chloroform solvent fractionation; and P4: methanol solvent fractionation, while the groups were the concentration of the fractions compound, which were Kl: 0 mg/ml; K2: 2.5 mg/ml; K3: 5 mg/ml and K4: 10 mg/ ml. The highest of the number of colonies of bacteria after contaminated with AFBl was obtained by GE with methcnol solvent at 2.5 rng/ml. The second phase, one hundred forty-four day old chicks were used in this experiment until 42 days old. They were randomly and equally divided into sixteen treatments. Each treatment had nine broilers and were arranged in factorial design and continued with Duncan's New Multiple Range Test. Factor A were the level of AFB1: A1 : 0 ppb (control); A2: 500 ppb; A3: 1000 ppb; and A4: 1500 ppb, then factor B were the level of GE: B1: 0% (control); B2: 2%; 83: -! %; and B-!: 6%. Aflatoxin Bl was given by oral and GE was given in rations. The variables observed were feed consumption, body weight, feed conversion, carcass weight, the percentage of abdominal fat, level of cholesterol serum, level of low density Lipoprotein (LDL) serum, level of high density lipoprotein (HDL) serum, liver weight, bursa of fabricius weight, the percentage of abdominal fat, level of glutamine pyruvat h·ansaminase serum (SGPT) and residues of AFBt on broiler liver and meat. Data collection was carried out twice, on 21 and 42 days. The results of the experiment showed AFB1 500 ppb increased): feed conversion, liver weight, body weight, level of SGPT and the percentage of abdominal fat (21 days), and reduced level of HDL serum and bursa of fabricius weight (42 days) significantly (P