Diabetes mellitus adalah gangguan metabolik yang ditandai dengan kondisi hiperglikemia, dapat disebabkan oleh gangguan fungsi pankreas dalam memproduksi insulin. Salah satu alternatif pengendalian hiperglikemia dengan pemanfaatan bahan alami yang berasal dari tanaman yang diduga mengandung zat hipoglikemik seperti daun Sansevieria trifasciata Prain yang banyak digunakan masyarakat Sabah, Malaysia. Penelitian ini bertujuan untuk mengetahui pengaruh rebusan daun lidah mertua (Sansevieria trifasciata Prain.) terhadap kadar glukosa darah, mikroanatomi pankreas tikus putih (Rattus norvegicus L.) yang menderita diabetes mellitus, serta menguji tingkat keamanannya melalui pengukuran kadar SGPT dan SGOT. Penelitian ini dilakukan dengan rancangan acak lengkap menggunakan tikus jantan, galur Wistar, umur 2 bulan, berat badan 150-200 gram selama 30 hari. Dua puluh lima ekor tikus dibuat hiperglikemik menggunakan aloksan monohidrat dosis tunggal 130 mg/kg bb secara intraperitonial. Tiga puluh ekor tikus dibagi menjadi 6 kelompok, yaitu (1) kelompok kontrol normal (2) kontrol diabetik diberi aquades, (3) kontrol obat diberikan glibenklamid dosis 5 mg/kgbb, (4,5,6) kelompok perlakuan diberikan rebusan daun lidah mertua dosis 100 mg/kgBB,150 mg/kgBB, dan 200 mg/kgBB. Preparat mikroanatomi pankreas diwarnai mengunakan pewarnaan Hemaktoksilin&Eosin, dan Victoria Blue. Hasil penelitian dianalisis menggunakan ANOVA dan LSD (p<0,05), preparat mikroanatomi dianalisis secara deskriptif dan semikuantitatif. Hasil penelitian selama 30 hari menunjukkan pemberian rebusan daun lidah mertua dengan dosis 100mg/kgBB dan 150 mg/kgBB efektif menurunkan kadar glukosa. Rebusan daun lidah mertua juga dapat meningkatkan kerapatan granula sel β pulau Langerhans pankreas tikus putih diabetik aloksan.

Diabetes mellitus is a metabolic disorder characterized by hyperglycemia conditions. It can be caused by impaired function of the pancreas in producing insulin. One alternative to control hyperglycemia is the use of natural ingredients derived from plants, which is suspected to contain hypoglycemic agents such as Sansevieria trifasciata Prain. leaf. The leaf is widely used in Sabah community, Malaysia. The purpose of this experiment is to examine the effect of decoction Sansevieria trifasciata Prain on blood glucose levels, microanatomy of pancreas from hyperglycemic rats (Rattus norvegicus L.) which are induced by alloxan, and to test its side effect by measuring levels of ALT and AST. This experiment was done by using complete randomized design, 30 male rats Wistar strain at 2 months, 150-200 gram BW in 30 days. Twenty-five male rats were induced by alloxan monohydrate single dose of 130 mg/kgbw intraperitoneally. Then thirty rats were divided into 6 groups, (1) normal control, (2) diabetic control given aquades, (3) control of the drug given glibenclamide 5 mg/kgbw, (4,5,6) treatment group: given leaf decoction Sansevieria trifasciata Prain respectively 100 mg/kgbw, 150 mg/kgbw, and 200 mg/kgbw. Microanatomy of pancreas was observed by using Haematoxylins-Eosin and Victoria Blue staining. The results were analyzed using ANOVA and LSD (p <0.05), slide microanatomy were analyzed descriptively and semi-quantitatively. The results show that the treatment leaf decoction 100mg/kgBW and 150 mg/kgBW most effectively decreases blood glucose levels. Decoction from Sansevieria trifasciata Prain leaf can also increase density of granule β cells of pancreatic Langerhans islets of alloxan diabetic rats.

Kata Kunci : Sansevieria trifasciata Prain,Glukosa darah,Sel beta pankreas,SGPT,SGOT,Aloksan monohidrat