Latar Belakang : Di tahun 2018, 10,9% penduduk Indonesia diatas usia 15 tahun mengidap diabetes melitus dan komplikasinya. Penderita diabetes melitus beresiko mengalami ulkus, dan lendir A. fulica diduga dapat membantu proses penyembuhan luka. Tujuan Penelitian: mengetahui pengaruh lendir A. fulica terhadap ekspresi gen bFGF dan VEGF pada proses penyembuhan luka eksisi kulit tikus diabetik yang diinduksi streptozotocin. Metodologi Penelitian: Penelitian ini adalah penelitian eksperimental murni, post-test only control group design. Subyek penelitian adalah 15 ekor tikus putih galur Wistar (Rattus norvegicus), yang dibagi menjadi 3 kelompok, yaitu kelompok kontrol positif, kontrol negatif dan perlakuan. Pada kelompok kontrol negatif luka tidak diberi obat, kontrol positif diberi gel ekstrak plasenta 10% + neomisin sulfat 0.5% dan perlakuan diberi gel ekstrak lendir A. fulica kadar 10%. Pengumpulan data dimulai dengan mengekstraksi RNA sampel area perlukaan, kemudian setelah melalui proses RT-PCR dan elektroforesis, didapatkan data berupa densitas pita/ekspresi gen bFGF dan VEGF. Hasil Penelitian : Ekspresi bFGF pada kelompok perlakuan lebih tinggi dibandingkan dengan kelompok kontrol positif dan kontrol negatif, namun hanya perbandingan terhadap kontrol negatif yang bermakna secara statistik (p<0,05). Ekspresi VEGF pada kelompok perlakuan lebih tinggi dibandingkan dengan kontrol positif maupun kontrol negatif yang tidak bermakna secara statistik (p>0,05). Kesimpulan: Pemberian lendir A. fulica memacu ekspresi bFGF yang lebih tinggipada proses penyembuhan luka eksisi kulit tikus diabetik yang diinduksi streptozotocin, dibandingkan dengan kelompok yang tidak diberi obat.

Background: In 2018, 10.9% of Indonesia's population over the age of 15 had diabetes mellitus and its complications. People with diabetes mellitus are at risk for ulcers, and A. fulica's mucus is promising in helping the wound healing process. Objective: To determine the effect of A. fulica's mucus on the expression of bFGF and VEGF genes in the excisional wound healing process of streptozotocin induced diabetic rat skin. Methodology: This research is an experimental research, post-test only control group design. The subjects of the study were 15 Wistar (Rattus norvegicus) strain white rats, which were divided into 3 groups, namely the positive control group, negative control and treatment. In the negative control group the wound was not given any drug, positive control was given 10% placenta extract gel + 0.5% neomycin sulfate and the treatment group was given 10% A. fulica's mucus extract. Data collection was started by extracting RNA samples from wound sites, then after going through the RT-PCR process and electrophoresis, data were obtained in the form of band density/bFGF and VEGF gene expression. Results: The expression of bFGF in the treatment group was higher than the positive control group and negative control, but only the comparison with negative controls was statistically significant (p <0.05). VEGF expression in the treatment group was higher than the positive control and negative control which was not statistically significant (p> 0.05). Conclusions: Topical application of A. fulica's mucus stimulates higher bFGF ex-pression in the excisional wound healing process of streptozotocin induced diabetic rat skin.

Kata Kunci : Achatina fulica, bFGF, diabetes mellitus, diabetic ulcer, wound healing, VEGF