GAMBAR HISTOPATOLOGI OTAK TIKUS YANG DI INDUKSI MONOSODIUM GLUTAMAT (MSG) DENGAN PEMBERIAN JAMUR KANCING (Agaricus bisporus)

FIRARR AMALLIA, Prof. Dr. drh. Siti Isrina Oktavia Salasia

2016 | Skripsi | S1 KEDOKTERAN HEWAN

Monosodium glutamat (MSG) adalah salah satu bahan penyedap masakan dan banyak digunakan untuk merangsang selera makan yang ditambahkan kedalam masakan. Pengunaan monosodium glutamat dalam jangka waktu yang lama dapat mengakibatkan adanya akumulasi glutamat sehingga menimbulkan eksitotoksin. Pemberian jamur kancing diduga dapat mengurangi efek eksitotoksin karena jamur kancing memiliki antioksidan tinggi. Tujuan dari penelitian adalah untuk mengetahui potensi jamur kancing sebagai antioksidan dalam mengurangi efek monosodium glutamat. Penelitian menggunakan 35 ekor tikus jantan (Rattus norvegicus) strain Sprague Dawley umur 1,5 bulan, diadaptasikan dengan lingkungan selama satu minggu, kemudian dibagi menjadi tujuh kelompok (4 kontrol dan 3 perlakuan) secara acak. Kelompok kontrol diberi larutan (NaCl fisiologis, kelompok K1 diberi MSG 8g/kg BB (6 hari) dan NaCl fisiologis (hari 7-35), kelompok K2 diberi MSG 8g/kg BB (12 hari) dan NaCl fisiologis (hari 13-35), kelompok K3 diberi MSG 8g/kg BB (24 hari) dan NaCl fisiologis (hari 25-35), kelompok P1 diberi MSG 8g/kg BB (6 hari) dan ekstrak jamur kancing 0,12g/200g BB (hari 7-35), kelompok P2 yang diberi MSG 8g/kg BB (12 hari) dan ekstrak jamur kancing 0,12g/200g BB (hari 13-35), kelompok P3 diberi MSG 8g/kg BB (24 hari) dan ekstrak jamur kancing 0,12 g/200g BB (hari 25-35). Hasil penelitian menunjukkan bahwa induksi monosodium glutamat kelompok K1, K2, dan K3 memperlihatkan adanya degenerasi neuron pada bagian hipokampus otak tikus. Induksi MSG yang disertai dengan pemberian ekstrak jamur kancing pada kelompok P1, P2 dan P3 ternyata juga memperlihatkan adanya degenerasi neuron. Dari hasil penelitian dapat disimpulkan bahwa jamur kancing belum dapat mengatasi kerusakan neuron akibat induksi monosodium glutamat.

Monosodium glutamate (MSG) is an ingredient that used to stimulate appetite. The use of MSG in the long term can lead to the accumulation of glutamate causing excitotoxins. The antioxidatif of the button mushrooms can be used to reduce the effects of excitotoxins. The purpose of the study was to determine the potensial of button mushrooms as antioxidants to neutralize the effects of MSG. The study used 35 male rats (Rattus norvegicus) Sprague Dawley strain 1,5 months of age were adapted to the environment for one week, and then were divided into seven groups (4 control and 3 treatment group). Control group, the rats were given physiological saline solution, group K1 by MSG 8g/kg BB (6 days) and physiological saline solution (days7-35), group K2 rats were given MSG 8g/kg BB (12 days) and physiological saline solution (days 13-35), the group K3 by MSG 8g/kg BB (24 days) and physiological saline solution (days 25-35), P1 group was given MSG 8g/kg BB (6 days) and button mushroom extract 0,12g/200g BB (days 7-35), group P2 by MSG 8g/kg BB (12 days) and button mushroom extract 0,12g/200g BB (days 13-35), group P3 by MSG 8G/KG BB (25 days) and button mushroom extract 0,12g/200g BB (days 25-35). The results showed that the induction of MSG of the rats in the group K1,K2, and K3 showed degeneration of neurons in the hippocampus of the brain of rats. Induction MSG that followed by the extract button mushrooms in the group P1,P2, and P3 showed also the degeneration of neurons. From the results of this study could be concluded that the button mushrooms can not overcome the neuronal damage induced by MSG.

Kata Kunci : Button Mushrooms (Agaricus bisporus), MSG, hippocampus.