Pengaruh Salinitas dan Media Limbah Cair Tahu terhadap Pertumbuhan, Lipid, Protein, Karbohidrat dan Pigmen Euglena sp.

AHMAD SAIFUN NASER, Dr. Eko Agus Suyono, S.Si., M.App.Sc.

2022 | Tesis | MAGISTER BIOLOGI

Industri tahu yang dimulai dari skala rumahan hingga pabrikan masih banyak mengalirkan limbah cair tahu ke sungai. Limbah cair tahu mengandung berbagai nutrisi esensial seperti P, N dan C dimana memiliki potensi sebagai medium kultur mikroalga Euglena sp. Adapun salinitas memiliki efek dalam fisiologi dan biokimia mikroalga. Penelitian ini bertujuan untuk menentukan pengaruh penambahan limbah cair tahu 0% (CM), 75% (MI) dan 100% (LO) dengan berbagai konsentrasi salinitas 0 g L⁻¹ (0), 2 g L⁻¹ (2) dan 4 g L⁻¹ terhadap kepadatan sel, biomassa, total lipid (Bligh and Dyar method), karbohidrat (phenol-sulfuric acid method), Total protein (Bradford method), klorofil A, klorofil B dan karotenoid pada Euglena sp. Berdasarkan penelitian, gabungan limbah cair tahu dan salinitas mampu meningkatkan kepadatan sel tertinggi yaitu 17,625 x 10⁵± 0.125 x 10⁵ sel/mL (MI2), Biomassa 2.77 ± 0.118 mg/mL (MI2), total lipid yaitu 0.81 ± 0.020 mg/mL (LO4), total karbohidrat yaitu 0.48 ± 0.014 mg/mL (MI4), total protein yaitu 0.14 ± 0.005 mg/mL (MI4), Klorofil A yaitu 10.74 ± 0.14 mg/L (LO4), Klorofil B yaitu 2.53 ± 0.07 mg/L (LO4) dan karotenoid yaitu 2.57 ± 0.003 mg/L (LO4). Berdasarkan uji Friedman's two-way ANOVA Penambahan limbah cair tahu dan salinitas berdampak signifikan terhadap masing masing pengujian,sehingga dapat disimpulkan bahwa limbah cair tahu berpotensi sebagai medium alternatif yang murah, pada Euglena sp. Namun, pengoptimalan antara limbah cair tahu dan salinitas harus sesuai dengan tujuan kultivasi.

The tofu industry, which starts from a home scale to manufacturers, still flows a lot of tofu liquid waste into rivers. Tofu liquid waste contains various essential nutrients such as P, N, and C, which have the potential as a culture medium for microalgae Euglena sp. Salinity affects the physiology and biochemistry of microalgae. This study aims to determine the effect of adding liquid tofu waste 0% (CM), 75% (MI), and 100% (LO) with various concentrations of salinity 0 g L⁻¹ (0), 2 g L⁻¹ (2), and 4 g L⁻¹ (4) on cell density, biomass, total lipid (Bligh and Dyar method), carbohydrates (phenol-sulfuric acid method), total protein (Bradford method), chlorophyll A, chlorophyll B and carotenoids in Euglena sp. Based on the research, the combination of tofu wastewater and salinity was able to increase the highest cell density, namely 17.625 x 10⁵ ± 0.125 x 10⁵ cells/mL (MI2), Biomass 2.77 ± 0.118 mg/mL (MI2), total lipid 0.81 ± 0.020 mg/mL (LO4), total carbohydrate was 0.48 ± 0.014 mg/mL (MI4), total protein was 0.14 ± 0.005 mg/mL (MI4), chlorophyll A was 10.74 ± 0.14 mg/L (LO4), chlorophyll B was 2.53 ± 0.07 mg/L (LO4) and carotenoids were 2.57 ± 0.003 mg/L (LO4). Based on Friedman's two-way ANOVA test, the addition of tofu liquid waste and salinity had a significant impact on each test, so it can be concluded that tofu liquid waste has the potential as a cheap alternative medium in Euglena sp. However, the optimization between tofu liquid waste and salinity must be under the cultivation objectives.

Kata Kunci : Euglena sp., Limbah Cair Tahu, Metabolit primer, pigmen, Salinitas