Perubahan Kadar Tissue Inhibitor Metalloproteinase-1 pada Pasien Astigmatisme Ringan-Sedang Sebelum dan Setelah Dilakukan Terapi Collagen Cross-Linking Kornea

BAYU PRIMAHATMAJA, Prof. dr. Suhardjo, SU., Sp.M(K).

2022 | Tesis-Spesialis | ILMU KESEHATAN MATA

Latar Belakang Untuk mengetahui perubahan kadar Tissue Inhibitor Metalloproteinase-1 (TIMP-1) sebelum dan setelah terapi Collagen Cross-Linking (CXL) pada pasien astigmatisme ringan-sedang. Metode Penelitian ini merupakan one group pre and post test. Total 10 pasien diperiksa status astigmat dan kondisi anatomi mata. Kadar TIMP-1 diambil dari air mata sebelum CXL, 7 hari, 30 hari dan 90 hari setelah terapi lalu diperiksa menggunakan metode ELISA. Hasil Semua sampel menunjukkan penurunan kadar TIMP-1 dalam 90 hari follow up setelah CXL (p=0,00). Ditemukan perbaikan manifest refraction spherical equivalent (p=0,00), perbaikan UCVA (p=0,00), peningkatan central corneal thickness (p=0,00) dan tidak ditemukan perubahan anatomi pada mata. Kesimpulan Pada penelitian ini didapatkan adanya penurunan kadar TIMP-1 pada pasien dengan astigmatisme ringan sedang disertai perbaikan kondisi klinis setelah CXL.

Purpose To determine the change of Tissue Inhibitor Metalloprotein-1 (TIMP-1) before and after Collagen Cross Linking Therapy (CXL) in patient with mild moderate astigmatism. Methods This was one group pre and post test examination. A Total 10 patients were enrolled in this study and were examined astigmatism status and eye anatomy. The TIMP-1 was extracted from tears before CXL, 7th day after therapy, 30th day after therapy and 90 day after therapy, then examined using ELISA. Results All samples showed decrease of TIMP-1 expression 90 day after CXL (p=0,00). There were improvement of manifest refraction spherical equivalent (p=0,00), improvement of UCVA (p=0,00), increase central corneal thickness (p=0,00) and no change in posterior anatomy of the eye. Conclusions There was decreasing expression of TIMP-1 in patient with mild moderate astigmatism with improvement of clinical condition after CXL.

Kata Kunci : TIMP-1, Collagen Cross Linking, CXL