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Pengaruh Pemberian Ubi Jalar Ungu (Ipomoea Batatas Var Antin 3) dan Vitamin E terhadap Catalase, Gluthatione Peroxidase, dan TNF-alfa dari Serum Darah Tikus dengan Overtraining Exercise

SITI KHADIJAH, dr. Jajar Setyawan, M.Sc., Ph.D.; Dr. Dwi Aris Agung Nugrahaningsih, M.Sc., Ph.D

2022 | Tesis | MAGISTER ILMU BIOMEDIK

Latar belakang: overtraining exercise adalah latihan fisik berlebihan sehingga membentuk Reactive Oxigen Species (ROS). Pencegahan dengan ubi jalar ungu (Ipomoea Batatas Var Antin 3) yang mengandung antosianin dan vitamin E (tokoferol) yang dapat meningkatkan fungsi mitokondria dan penekanan sitokin pro-inflamasi. Tujuan: mengetahui perbedaan efek pemberian ubi jalar ungu dan Vitamin E terhadap kadar CAT (catalase), GPx (glutathione peroxidase), dan TNF-alfa (tumor necrosis alpha) dari serum darah tikus. Metode: jenis penelitian true experimental secara post test only control group design (n= 25 ekor Tikus Galur Wistar). Semua kelompok diberikan aktivitas prakondisi yaitu renang. K1 ialah kelompok kontrol. K2 ialah kelompok perlakuan (overtraining ecercise). K3 ialah pemberin ubi jalar ungu dosis 0,63 g/ekor/hari. K4 ialah pemberian ubi jalar ungu dosis 1,26 g/ekor/hari. K5 ialah vitamin E 1,44 mg. Analisis data menggunakan uji one way anova. Hasil: rerata Kadar CAT K1= 5,86+-0,19, K2= 1,73+-0,04, K3= 3,43+-0,07, K4= 4,40+-0,08, K5= 5,34+-0,14. Hasil analisis dengan tes anova yaitu p=0,000. Secara statistik terdapat perbedaan rerata kadar CAT dengan tes post hoc tamhanes K4 VS K5 p= 0,000. Rerata kadar GPx pada K1= 71,30+-2,08, K2= 22,22+-2,14, K3= 49,69+-2,08, K4= 60,04+-2,20, K5= 70,38+-1,84. Hasil analisis dengan tes anova yaiutu p= 0,000. Secara statistik terdapat perbedaan rerata kadar GPx dengan tes Post Ho c LSD K4 VS K5 p= 0,000. Rerata kadar TNF-alfa pada K1= 6,85+-0,59, K2= 22,97+-1,17, K3= 11,15+-0,81, K4= 8,37+-0,48, K5= 8,02+-0,67. Hasil analisis dengan tes anova p=0,000. Tidak terdapat perbedaan rerata kadar TNF-alfa dengan tes Post Hoc LSD K4 VS K5 p= 0,481. Kesimpulan: Ada perbedaan efek pemberian ubi jalar ungu dosis sedang dan tinggi dibandingkan vitamin E dalam rerata kadar CAT dan GPx yang lebih tinggi pada Tikus Galur Wistar yang diberikan perlakuan overtraining exercise. Kemudian, hanya terdapat perbedaan efek pemberian ubi jalar ungu dosis sedang, dan pada dosis tinggi tidak terdapat perbedaan efek dibandingkan vitamin E dalam rerata kadar TNF-alfa.

Background: overtraining exercise is excessive physical exercise to form Reactive Oxygen Species (ROS). Prevention with purple sweet potato (Ipomoea Batatas Var Antin 3) which contains anthocyanins and vitamin E (tocopherol) which can improve mitochondrial function and suppress pro-inflammatory cytokines. Objective: determine the difference in the effect of Ipomoea Batatas Var Antin 3 (purple sweet potato) and Vitamin E on the levels of CAT (catalase), GPx (glutathione peroxidase, and TNF-alfa (tumor necrosis alpha) from rat blood serum. Method: true experimental type of research with post test only control group design (n= 25 Wistar rats). All groups were given a precondition activity, namely swimming. K1 is the control group. K2 is the treatment group (overtraining ecercise). K3 is giving purple sweet potato at a dose of 0.63 g/head/day. K4 is the administration of purple sweet potato at a dose of 1.26 g/head/day. K5 is vitamin E 1.44 mg. Data analysis used one way ANOVA test. Results: Mean CAT levels K1= 5.86+-0.19, K2= 1.73+-0.04, K3= 3.43+-0.07, K4= 4.40+-0.08, K5= 5.34+-0 ,14. The results of the analysis with the ANOVA test are p = 0.000. Statistically there was a difference in the mean CAT levels with the post hoc Tamhanes K4 VS K5 test p = 0.000. The mean GPx levels at K1= 71.30+-2.08, K2= 22.22+-2.14, K3= 49.69+-2.08, K4= 60.04+-2.20, K5= 70.38+- 1.84. The results of the analysis with the ANOVA test are p= 0.000. Statistically there was a difference in the mean GPx levels with the Post Hoc LSD K4 VS K5 test, p= 0.000. The mean levels of TNF-alfa at K1= 6.85+-0.59, K2= 22.97+-1.17, K3= 11.15+-0.81, K4= 8.37+-0.48, K5= 8, 02+-0.67. The results of the analysis with the ANOVA test p= 0.000. There was no difference in the mean levels of TNF-alfa with the Post Hoc LSD K4 VS K5 test, p= 0.481. Conclusion: There was a difference in the effect of giving medium and high doses of purple sweet potato compared to vitamin E in the higher mean levels of CAT and GPx in Wistar Strain rats given overtraining exercise treatment. Then, there was only a difference in the effect of medium-dose purple sweet potato, and at high doses there was no difference in effect compared to vitamin E in the mean levels of TNF-alfa.

Kata Kunci : Antosianin, Catalase, glutathione peroxidase, TNF-alfa, overtraining exercise, ubi jalar ungu, tokoferol, Vitamin E

  1. S2-2022-465972-abstract.pdf  
  2. S2-2022-465972-bibliography.pdf  
  3. S2-2022-465972-tableofcontent.pdf  
  4. S2-2022-465972-title.pdf