PENGARUH VARIASI KONSENTRASI TETRAHIDROPENTAGAMAVUNON-5 (THPGV-5) DALAM SEDIAAN LOTION TERHADAP NILAI SUN PROTECTING FACTOR (SPF) SECARA IN VITRO DAN EFEK IRITASI AKUT DERMAL

GABRIELLA M D P, Dr. Ritmaleni, S.Si ; apt. Angi Nadya Bestari, M.Sc

2020 | Skripsi | S1 FARMASI

Tetrahidopentagamavunon-5 (THPGV-5) merupakan senyawa analog kurkumin yang memiliki aktivitas antioksidan yang lebih baik dari vitamin E. Lotion THPGV-5 telah diformulasikan sebelumnya namun belum dilakukan penentuan nilai SPF dan efek iritasi akut dermalnya. Penelitian ini bertujuan untuk mengetahui pengaruh variasi konsentrasi THPGV-5 dalam sediaan lotion terhadap efek iritasi akut dermal dan memprediksi nilai sun protecting factor (SPF) secara in vitro. Dalam penelitian ini dilakukan formulasi lotion THPGV-5 dan diuji sifat fisiknya yang meliputi organoleptis, homogenitas, pH, daya lekat dan daya sebar. Penentuan nilai SPF dilakukan secara in vitro dengan menggunakan spektrofotometer UV-Vis dan dibaca absorbansinya pada panjang gelombang 290-320 nm. Absorbansi dibaca setiap selisih panjang gelombang 5 nm. Nilai absorbansi kemudian dihitung menggunakan persamaan Mansur dan diuji secara statistik One-Way ANOVA. Dikarenakan adanya pandemik COVID-19 sehingga laboratorium tidak dapat digunakan, maka pengujian nilai SPF dilakukan dengan metode review literatur terhadap artikel dengan penentuan nilai SPF senyawa golongan kurkumin. Dilakukan uji iriasi akut dermal secara in vivo menggunakan kelinci sesuai dengan Pedoman Uji Toksisitas Nonklinik secara in vivo dari Badan Pengawasan Obat dan Makanan RI. Lotion THPGV-5 dengan konsentrasi 0,1%; 0,2% dan 0,4% menghasilkan indeks iritasi 0; 0,0139 dan 0,0139 dimana tergolong dalam sediaan yang mengiritasi sangat ringan (negligible). Nilai SPF dengan bahan aktif dari golongan kurkumin seperti Curcuma manga dan Curcuma longa dengan peningkatan konsentrasi menghasilkan nilai SPF yang semakin tinggi maka semakin tinggi konsentrasi THPGV-5 akan semakin meningkatkan nilai SPF. Berdasarkan hasil penelitian dapat disimpulkan bahwa kenaikan konsentrasi THPGV-5 dalam lotion tidak mempengaruhi efek iritasi dan dapat meningkatkan nilai SPF.

Tetrahidopentagamavunon-5 (THPGV-5) is a curcumin analogue that has better antioxidant activity than vitamin E. The THPGV-5 lotion has been formulated before but the SPF value and its acute irritating effect have not been determined. This study aims to determine the effect of variation THPGV-5 concentration in lotion toward the effects of acute dermal irritation and to predict the value of sun protecting factor (SPF) in vitro study. In this study a THPGV-5 lotion formulation was performed and its physical properties were tested which included organoleptic, homogeneity, pH, adhesion and spreadability. Determination of the SPF value was done in vitro using a UV-Vis spectrophotometer and the absorbance was read at a wavelength of 290-320 nm. Absorbance is read every 5 nm wavelength difference. The absorbance value then calculated using the Mansur equation and statistically tested with One-Way ANOVA. Due to the existence of a COVID-19 pandemic so that the laboratory cannot be used, the SPF value testing was carried out by the literature review method of the article by determining the SPF value of curcumin group compounds. Acute dermal irritation was performed in vivo using rabbits in accordance with the Non-Clinical Toxicity Test Guidelines from the Indonesia National Food and Drug Agency. Lotion THPGV-5 with a concentration of 0.1%; 0.2% and 0.4% produce an irritation index of 0; 0.0139 and 0.0139 which are classified as highly negligible irritants. SPF values with active ingredients from the curcumin group such as Curcuma manga and Curcuma longa with increasing concentrations produce higher SPF values, the higher the THPGV-5 concentration will further increase the SPF value. Based on the results of the study it can be concluded that the increase in THPGV-5 concentration in the lotion does not affect the irritating effect and can increase the SPF value.

Kata Kunci : THPGV-5, Lotion, Sun Protecting Factor (SPF), Iritasi / THPGV-5, Lotion, Sun Protecting Factor (SPF), Irritation