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Pengaruh Pemberian Probiotik secara Oral terhadap Pertahanan Tubuh Non-spesifik Humoral Ikan Lele (Clarias sp.)

NYI JAYANTI, Dr. Ir. Alim Isnansetyo, M.Sc.

2019 | Skripsi | S1 AKUAKULTUR

Probiotik merupakan biakan mikroba yang menguntungkan bagi inang. Penelitian ini bertujuan untuk mengetahui pengaruh dan dosis efektif pemberian probiotik pada pakan buatan terhadap pertahanan tubuh non-spesifik humoral Clarias sp. Probiotik uji terdiri atas tiga jenis bakteri, yaitu Bacillus sp. T2A, Bacillus sp. T3PI, dan Lactococcus raffinolactis JALI. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 3 ulangan. Perlakuan yang diberikan yaitu kontrol negatif (pakan tanpa probiotik) (P1), pakan ditambah probiotik dosis 102 CFU/g (P2), pakan ditambah probiotik dosis 103 CFU/g (P3), pakan ditambah probiotik dosis 104 CFU/g (P4), dan kontrol positif (pakan buatan ditambah probiotik komersial merk Raja Lele dengan dosis 7,5 ml/kg) (P5). Pemberian pakan dilakukan 2 kali sehari dengan dosis 3% dari biomassa ikan uji. Pengambilan sampel darah dilakukan pada hari ke 0, 14, 28, 42, dan 56. Parameter pertahanan tubuh non-spesifik humoral yang diamati yakni aktivitas antibakteri serum, aglutinasi alami, dan total protein plasma (TPP). Sedangkan leukokrit dan hematokrit diamati untuk mengetahui parameter darah dalam kondisi normal. Hasil penelitian menunjukkan bahwa pemberian probiotik dapat meningkatkan pertahanan tubuh non-spesifik humoral Clarias sp. melalui peningkatan persentase aktivitas antibakteri serum. Dosis efektif pemberian probiotik untuk meningkatkan pertahanan tubuh non-spesifik humoral Clarias sp. berdasarkan penelitian yang dilakukan adalah 102 CFU/g.

Probiotics are microbial culture wich are beneficial to the host. The research aimed to determine the effect and the effective of probiotics dosage in commercial feed on Clarias humoral innate immune. The probiotics were composed of three bacteria, namely Bacillus sp. T2A, Bacillus sp. T3PI, and Lactococcus raffinolactis JALI. The experimental design used in this research was Randomized Complete Design (RCD) with 5 treatments and 3 replications. The treatments in this research consisted of negative control (without administration of probiotic) (P1), administration of probiotics at 102 CFU/g (P2), administration of probiotics at 103 CFU/g (P3), administration of probiotics at 104 CFU/g (P4), and a positive control (administration of �Raja Lele� probiotic 7,5 mL/kg dose. Clarias sp. was fed twice daily at 3% of its biomass. Blood samples were taken at day 0, 14th, 28th, 42th, and 56th. Parameters of humoral innate immune that observed were serum antimicrobial activity, natural aglutination, and total plasm a protein. The persentage of leucocyte and eritrocyte were observed to know the normal blood parameters. The results showed that administration of probiotics in feed significantly increase non specific humoral body defense of Clarias sp. by increasing the percentage of serum antibacterial activity. The effective dosage of probiotics administration to increase Clarias humoral innate immune according to this research was 102 CFU/g.

Kata Kunci : Clarias sp., humoral, pertahanan tubuh non-spesifik, probiotik

  1. S1-2019-363963-abstract.pdf  
  2. S1-2019-363963-bibliography.pdf  
  3. S1-2019-363963-tableofcontent.pdf  
  4. S1-2019-363963-title.pdf