ISOLASI DAN UJI ANTIOKSIDAN TERHADAP FRAKSI POLAR EKSTRAK SPONS CALTHROPELLA SP. DARI PANTAI KRAKAL, GUNUNG KIDUL; ISOLATION AND ANTIOXIDANT ASSAY OF POLAR FRACTION OF MARINE SPONGE CALTHROPELLA SP. EXTRACT FROM KRAKAL BEACH, GUNUNG KIDUL
GIOVANNO, ALDIAN, Swasono, Respati Tri
2015 | Skripsi | FMIPA UGMIsolation and identification of antioxidant compounds of marine sponge Calthropella sp. have been carried out. The aims of this research are to isolate, to test the antioxidant activity, and to identify the antioxidant compounds of polar fractions of Calthropella sp. from Krakal Beach, Gunung Kidul. Isolation was performed by maceration using a mixture of methanol : dichloromethane (2 : 1 v/v). Methanol extract was then partitioned into butanol. Methanol extract was separated with a reversed-phase column, Solid Phase Extraction (SPE) using aquabides, methanol 10%, 20%, 30%, 40%, 50%, and 60% as eluents. Crude extract and its fractions were tested for their antioxidant activities using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay. Fractions with high antioxidant activities were identified by Liquid Chromatography-Mass Spectroscopy (LC-MS). Calthropella sp. crude extract, methanol 10% fraction, and methanol 20% fraction showed a strong antioxidant activity againts DPPH radical, with IC50 values of 68.786 ppm, 77.118 ppm, and 55.472 ppm respectively. LC-MS analysis indicated that methanol 10% fraction contained curcuphenol, caerulsteroid, and puupehenone, while methanol 20% fraction contained halioxepine and puupehenone.
Kata Kunci : Calthropella sp.; Solid Phase Extraction; Antioxidant; DPPH assay
