ISOLASI, IDENTIFIKASI DAN UJI TOKSISITAS EKSTRAK KELINCI LAUT Aplysia parvula DENGAN METODE BRINE SHRIMP LETHALITY TEST (BST); ISOLATION, IDENTIFICATION AND TOXICITY TEST OF THE SEA HARE Aplysia parvula EXTRACT USING BRINE SHRIMP LETHALITY TEST (BST) METHOD

ANA ANDRIANA, Respati Tri Swasono

2013 | Disertasi | PROGRAM STUDI S2 ILMU KIMIA

Research on isolation, identification and toxicity test of the sea hare Aplysia parvula extract have been carried out. The isolation was performed by extraction process using a mixture of methanol:dichloromethane (2:1). Dichloromethane extract was than partitioned with ethyl acetat:water (3:2). Crude ethyl acetate extract was separated by column chromatohraphy using a gradient elution system of n-heksane, chloroform, ethyl acetate and methanol. Crude ethyl acetate extract and fractions from column chromatography were tested their toxicities using Brine Shrimp Lethality Test (BST) method. Compounds comprising the active fractions were analysed by Liquid Chromatography-Electrospray Ionisation Mass Spectrometry (LC-ESIMS). The result of toxicity test showed that Lethal Concentration 50 (LC50) value of ethyl acetate extract is 69,18 ?g/mL whereas fractions I - V from column chromatography are 330.37, 104.47, 295.80, 335.74, and 223.87 ?g/mL, respectively. LC-ESIMS analysis indicates that the fraction II consists of several compounds, i.e (1Z)-3-methyl-1-[(1S,6R)-2,2,6-trimethyl-7-oxabicyclo[4.1.0]hept -1-yl]penta-1,4-dien-3-yl acetate, cadalene, japonenyn-A, and eckstolonol. Compounds loliolide, japonenyne-A, and halogenated furanone vary likely comprises fraction V.

Kata Kunci : Aplysia parvula; toksisitas; Brine Shrimp Lethality Test (BST); ESI-MS