STUDI JENIS DAN DISTRIBUSI GLIKOKONJUGAT PADA LAMBUNG KERBAU RAWA (Bubalus bubalis) KALIMANTAN SELATAN DENGAN HISTOKIMIA LEKTIN
NURLIANI, Anni , Teguh Budipitojo
2009 | Tesis |Kemampuan kerbau rawa beradaptasi pada lingkungan rawa yang memiliki sumber daya pakan terbatas diduga didukung oleh faktor kemampuan efisiensi sistem pencernaannya. Penelitian ini dilakukan untuk mengkaji penjelasan ilmiah mengenai kemampuan efisiensi pencernaan kerbau rawa dengan mengidentifikasi jenis dan distribusi glikokonjugat pada daerah lambung kerbau rawa. Enam ekor kerbau rawa jantan, dewasa dengan umur > 2,5 tahun, berat badan 300-400 kg digunakan dalam penelitian ini. Sampel diperoleh dari RPH Kabupaten Banjar, Kalimantan Selatan, Indonesia. Setiap bagian lambung meliputi rumen, retikulum, omasum, dan abomasum (kardiak, fundus dan pilorus) diambil untuk pengamatan makroskopis, meliputi berat, panjang dan lebar organ. Kemudian seluruh sampel dari setiap bagian lambung diambil dan selanjutnya dilakukan pemrosesan jaringan untuk pengamatan mikroskopis dengan pewarnaan HE, AB-PAS dan untuk mendeteksi residu gula glikokonjugat pada lambung dengan pewarnaan histokimia lektin (WGA, UEA, RCA, Con A, dan SBA). Data yang diperoleh dianalisis secara deskriptif. Lambung kerbau rawa memiliki glikokonjugat D-N asetilglukosamin, L fukosa, D galaktosa, D manosa/D glukosa, dan D-N asetilglukosamin. Glikokonjugat D-N asetilglukosamin terdistribusi pada sel stratum basale daerah omasum; sel gastric pit dan seluruh kelenjar kardiak; sel gastric pit, sel leher mukus, dan sel parietal daerah fundus; serta sel gastric pit dan seluruh kelenjar pilorus. Glikokonjugat L fukosa teridentifikasi pada sel gastric pit, sel leher mukus, dan sel parietal daerah fundus; serta pada sel gastric pit dan seluruh kelenjar pilorus kerbau rawa. Glikokonjugat D galaktosa tersebar pada sel stratum korneum dan stratum basale daerah retikulum; sel stratum basale daerah omasum; sel mukus permukaan daerah kardiak; dan pada sel mukus permukaan, sel gastric pit, sel leher mukus, serta sel parietal daerah fundus kerbau rawa. Glikokonjugat D manosa/D glukosa ditemukan pada sel stratum korneum, stratum granulosum, stratum spinosum dan stratum basale daerah rumen, retikulum dan omasum; serta sel parietal daerah fundus kerbau rawa. Glikokonjugat D-N asetilgalaktosamin terdistribusi pada sel gastric pit, sel leher mukus, dan sel parietal daerah fundus kerbau rawa. Keberadaan glikokonjugat D manosa/D glukosa yang dominan pada lambung nonglandular diduga berperan penting dalam peningkatan efisiensi pencernaan kerbau rawa. Kata kunci : Kerbau rawa, glikokonjugat, lambung, histokimia lektin
The ability of swamp buffaloes to adapt with swamp environment was suggested to be supported by their digestive system efficiency. The research was done to obtain scientific explanation about digestive efficiency of swamp buffalo by identification on kind and distribution of glycoconjugates in swamp buffalo stomach. Six male swamp buffaloes (more than 2.5 years old and body weight between 300-400 kg were used in the study. Samples were obtained from Regency of Banjar slaughter house, South Kalimantan, Indonesia. Every parts of the stomach include rumen, reticulum, omasum, and abomasum (cardiac, fundus, and pylorus) was taken for macroscopic observation (i.e weight, length, and width organs). Then all of samples from each part of stomach were processed for microscopic observation with HE and AB-PAS staining. Sugar residues of glycoconjugates in stomach were localized with lectin histochemistry (WGA, UEA, RCA, Con A, and SBA). The data were analyzed descriptively. Swamp buffalo stomach has D-N-acetylglucosamine, L fucose, D galactose, D mannose/D glucose, and D-N acetylgalactosamine. Glycoconjugates D-N acetylglucosamine were distributed in cell of stratum basale omasum; gastric pit cell and all cardiac glands, gastric pit cell, mucous neck cell, and parietal cell fundic region; as well as gastric pit cell and all pyloric glands. Glycoconjugates L fucose were identified in gastric pit cell, mucous neck cell, and parietal cell fundic region; as well as gastric pit cell and all pyloric glands of swamp buffalo. Glycoconjugates D galactose were disseminated in cell of stratum corneum dan stratum basale reticulum; cell of stratum basale omasum; surface mucous cell cardiac region; as well as surface mucous cell, gastric pit cell, mucous neck cell, and parietal cell fundic region of swamp buffalo. Glycoconjugates D mannose/D glucose were found in cell of stratum corneum, stratum granulosum, stratum spinosum, dan stratum basale rumen, reticulum and omasum; as well as parietal cell fundic region of swamp buffalo. Glycoconjugates D-N acetylgalactosamine were identified in gastric pit cell, mucous neck cell, and parietal cell fundic region of swamp buffalo. Domination of glycoconjugates D mannose/D glucose in nonglanduler stomach was suggested to play important role in increase digestive efficiency of swamp buffalo stomach. Key words: Swamp buffalo, glycoconjugates, stomach, lectin histochemistry
Kata Kunci : Kerbau rawa, glikokonjugat, lambung, histokimia lektin
