PENGARUH PEMBERIANEKSTRAK ETANOL DAUN PEGAGAN(Centella asiatica (L.). Urb.) TERHADAPKADARBRAIN-DERIVED NEUROTROPHIC FACTOR(BDNF) DICEREBELLUM PADATIKUS PUTIH(Sprague Dawley) PASCASTRES LISTRIK KRONIK

Desby Juananda, dr. Dwi Cahyani Ratna Sari, M.Kes., PA(K).

2015 | Tesis | S2 Ilmu Kedokteran Dasar dan Biomedis

Latar Belakang:Stres kronik telah terbukti dapat menginduksi neurotoksisitas yang disebabkan oleh glukokortikoid, dan mempengaruhi kadar brain-derived neurotrophic factor (BDNF) sebagai neuroprotektor di otak. Cerebellum sangat rentan terhadap stres oksidatif terutama yang disebabkan oleh glukokortikoid. Efek neuroprotektif Centella asiatica terbukti dapat melindungi sel saraf dari stres oksidatif yang disebabkan oleh paparan stres kronik. Tujuan Penelitian:Mengungkapkan pengaruhekstrak etanol daun Centella asiatica terhadap kadar BDNF di cerebelum pada tikus putih pascastres listrik kronik. Metode:Dua puluh ekor tikus putih, galur Sprague Dawley, jantan, umur 2 bulan, berat badan 100-150 gram dibagi menjadi 4 kelompok secara random masing-masing 5 ekor tikus per kelompok. Kelompok stres hanya diberikan aquades, dan kelompok perlakuan CeA150, CeA300 dan CeA600 masing-masing diberikan ekstrak etanol daun Centella asiatica dengan dosis 150, 300 dan 600 mg/kgbb/hari per oral yang diikuti dengan paparan stres listrik kronik selama 28 hari. Pada akhir perlakuan, tikus dikorbankan dengan cara dekapitasi, kemudian cerebellum dipisahkan dari bagian otak lainnya. Kadar BDNF dari lisat jaringan cerebellum diukur dengan menggunakan metode ELISA. Data dianalisis dengan uji one-way ANOVA (p < 0,05). Hasil Penelitian:Rerata konsentrasi protein BDNF pada jaringan cerebellum (ρg/mL) masing-masing untuk kelompok stres, CeA150, CeA300 dan CeA600 adalah 1.217,10±301,40, 771,46±241,45, 757,05±268,29, 627,00±246,02 (p< 0,05). Uji post-hoc menunjukkan bahwa terdapat perbedaan bermakna antara kelompok stres dengan kelompok perlakuan (p <0,05), tetapiantara kelompok perlakuan tidak terdapat perbedaan bermakna (p> 0,05). Kesimpulan:Ekstrak etanol daun Centella asiaticamenurunkan kadar BDNF di cerebellum pada tikus putih pascastres kronik.

Background:Studies have shown that chronic stress can induce neurotoxicity caused by glucocorticoids, and alter concentration of brain-derived neurotrophic factor (BDNF) as a neuroprotective agent in the brain. Among brain regions, the cerebellum is known to be severely affected by oxidative damage associated with glucocorticoids. The neuroprotective effect of Centella asiatica has been reported to protect neurons from oxidative damagefollowingchronic exposure to variety stressors. Objective:To investigate the effect of Centella asiatica leaf ethanol extracts onrat cerebellar BDNF concentration following chronic footshock stress. Methods:Twenty young-adult (8 weeks old) male Sprague Dawley rats weighing 100- 150 g were randomly assigned into four experimental groups with five rats per group. Stresscontrol groupreceived aquadest, and other groups were treated with different doses (mg/kg body weight/day, p.o.) of Centella asiatica leaf ethanol extracts: 150 (CeA150), 300 (CeA300) and 600 (CeA600), respectively followed by chronic footshock stress for twenty-eight days. On completion of experimental period, animals were sacrificed by decapitation, and cerebellum was isolated from other brain tissue.BDNF concentrationfrom cerebellar tissue lysate was measured using ELISA method.Data were analyzed with one-way ANOVA (p< 0.05). Result: Mean BDNF concentration (ρg/mL) of cerebellar tissue for stress control, CeA150, CeA300 and CeA600 groups were 1217.10±301.40, 771.46±241.45, 757.05±268.29, 627.00±246.02, respectively(p<0,05).Post-hocanalysishad shown that there was significant difference between stress control group andgroups treated (p< 0.05), but there was no significant difference among group treated (p> 0.05). Conclusion: Ethanol extracts of Centella asiatica leaf decreasecerebellar BDNF concentration in rats following chronic stress.

Kata Kunci : BDNF, Cerebellum, Centella asiatica, Stres Kronik