PENGARUH EKSTRAK ETANOL KUNYIT (Curcuma longa L) TERHADAP EKSPRESI RESEPTOR LUTEINIZING HORMONE OVARIUM TIKUS STRAIN SPRAGUE DAWLEY

AKIKO SARASWATI A, Dr. dr. Rul Afiyah Syarif, M.Kes; Dr. Sri Herwiyanti, M.S; dr. Dwi Aris Agung Nugrahaningsih, M.Sc

2018 | Skripsi | S1 PENDIDIKAN DOKTER

Latar Belakang Peningkatan populasi telah menjadi salah satu isu penting yang memerlukan perhatian. Penurunan fertilitas terkait dengan keberhasilan pembangunan sosial dan ekonomi, khususnya di bidang Keluarga Berencana (KB). Salah satu tanaman yang berpotensi sebagai antifertilitas dan sering digunakan oleh masyarakat Indonesia adalah kunyit (Curcuma longa, Linn). Tujuan Mengetahui pengaruh ekstrak etanol kunyit (C. longa L.) terhadap ekspresi reseptor Luteinizing Hormone (LH) ovarium tikus strain Sprague Dawley. Metode Penelitian ini merupakan penelitian eksperimental laboratoris. Penelitian ini dilakukan pada tikus betina strain Sprague Dawley usia 6 minggu yang terbagi menjadi 4 kelompok, yaitu 1 kelompok kontrol dan 3 kelompok uji yang masing-masing diberi ekstrak etanol C. longa L dosis 200, 250, dan 300 mg/kgBB selama 8 siklus estrus. Setelah 8 minggu, tikus dibedah ketika fase proestrus dan diambil ovariumnya. Ekspresi reseptor LH folikel ovarium ditetapkan secara semikuantitatif setelah dilakukan pengecatan secara imunohistokimia. Data yang diperoleh diuji menggunakan uji ANAVA dan Kruskal-Wallis. Hasil Rerata skor ekspresi reseptor LH ovarium tikus strain Sprague Dawley kelompok perlakuan tidak mengalami penurunan dibandingkan dengan rerata skor ekspresi reseptor LH ovarium tikus strain Sprague Dawley kelompok kontrol. Hasil uji skor ekspresi reseptor LH pada semua folikel maupun tiap jenis folikel menunjukkan nilai signifikansi lebih dari 0,05 (p>0,05). Kesimpulan Ekstrak etanol kunyit (C. longa L) tidak menyebabkan penurunan ekspresi ovarium Luteinizing Hormone tikus strain Sprague Dawley.

Background The increase of population has become one of the important issues that need attention. The decline in fertility is related to the success of social and economic development, especially in the field of Family Planning. Turmeric (Curcuma longa, Linn) is a plant that is often used by Indonesians for contraceptive agent and has a potential antifertility. Objective To evaluate the potential antifertility of C. longa L ethanol extract on female Sprague Dawley rats by studying its effect on Luteinizing Hormone (LH) receptor expression in the ovary. Methods The rats used were Sprague Dawley of 6 weeks old. The rats were divided into 4 groups, 1 control group and 3 test groups treated with Curcuma longa L ethanol extract at doses of 200, 250, and 300 for 8 estrous cycles. After 8 weeks, the rats were dissected during the proestrus phase and the ovaries were taken to be made into preparations with immunohistochemistry staining. The expression of LH receptor of the ovarian follicles is determined using semiquantitative method. The data obtained were tested using ANOVA and Kruskal-Wallis tests. Results LH receptor expression of the treatment groups did not show lower mean scores compared to the mean scores of LH expression of the control group after the administration of C. longa L ethanol extract. The results of LH receptor expression score test on all follicles and each type of follicles showed no significant difference (p> 0.05). Conclusions The C. longa L ethanol extract does not reduce the expression of Luteinizing Hormone receptor in the ovary on Sprague Dawley rats.

Kata Kunci : ekstrak etanol Curcuma longa L, reseptor Luteinizing Hormone, Sprague Dawley