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DETEKSI KETERLIBATAN GENOME LARGE T-ANTIGEN SIMIAN VIRUS 40 SPESIFIK CYTOTOXIC T LYMPHOCYTE (CTL) EPITOPES I TERHADAP KASUS LIMFOMA NON-HODGKIN DI YOGYAKARTA

RESTANTI AULIA NOVITASARI, dr. Nungki Anggorowati, Ph.D, Sp.PA(K), dr. Ahmad Ghozali, Sp.PA(K)

2018 | Skripsi | S1 PENDIDIKAN DOKTER

Latar Belakang: Berdasarkan data statistik dari Rumah Sakit Pusat Kanker Dharmais tahun 2006, dilaporkan bahwa Limfoma Non-Hodgkin masuk dalam 10 diagnosis kanker tersering di Indonesia. Penelitian terdahulu menemukan adanya sekuensing SV40 positif pada mayoritas kasus Limfoma tipe NHL. Tujuan Penelitian: Mengetahui frekuensi keterlibatan genome L-Tag SV40 pada kasus NHL di Yogyakarta yang dihubungkan dengan tampakan klinik dan keragaman varian mutasi epitope L-Tag pada sekuensing SV40. Metode Penelitian: Preparat parafin yang telah terdiagnosis NHL diekstraksi DNA menggunakan metode Qiagen. Hasil ekstraksi digunakan untuk PCR sebanyak 2 kali. Primer memakai regio yang sesuai dengan regio target L-Tag dari SV40 spesifik sel T limfosit sitotoksik epitope I. Primer yang menjadi fokus adalah STEV189 dan STEV219. Sekuensing DNA untuk menemukan hasil. Hasil: Data pasien yang menderita limfoma non-hodgkin memiliki rentang usia 2 tahun hingga 87 tahun dengan nilai rata rata 50,93 dan nilai tengah 54,5. Sejumlah 54 sampel (53,46%) adalah pasien laki-laki dan 46 sampel (45,54%) adalah pasien perempuan, 1 (0,99%) sampel lain tidak terdapat data. Sejumlah 43 sampel (42,39%) dari intranodal dan 56 sampel (55,43%) dari ekstranodal, sedangkan 2 sampel (2,17%) tidak terdapat data. Hasil PCR dari genome L-Tag pada 101 sampel didapatkan hasil negatif pada seluruh sampel. Dengan tidak adanya hasil PCR yang bernilai positif, maka uji chi square untuk menentukan hubungan tidak dapat dilakukan Kesimpulan Tidak didapatkan hubungan antara genome Large T-Antigen Simian Virus 40 spesifik Cytotoxic T Lymphocyte (CTL) epitope I dan prevalensi kasus limfoma Non-Hodgkin di Yogyakarta.

Background: Based on statistical data from Dharmais Cancer Center Hospital in 2006, it was reported that Non-Hodgkin's Lymphoma is included in 10 most common cancer diagnoses in Indonesia. Some of previous studies have found positive SV40 sequencing in the majority of cases of Non Hodgkin's Lymphoma. Research Objectives: The aim of this study is to know the frequency of L-Tag SV40 genome involvement in NHL cases in Yogyakarta associated with clinical appearance and variation of L-Tag epitope mutation in SV40 sequencing. Research Methods: Paraffin blocks diagnosed with NHL were DNA extracted using the Qiagen method. The extraction results were used for PCR. Primers were used for the regions corresponding to the L-Tag target region of the specific SV40 T cells of epitope I cytotoxic lymphocytes. Primary focuses were STEV189 and STEV219. DNA sequencing was performed to find results. Results: Data for patients with Non-Hodgkin's Lymphoma ranged from 2 years to 87 years with an average score of 50.93 and a mean of 54.5. Total of 54 samples (53.46%) were male and 46 samples (45.54%) were female, 1 sample (0.99%) was not found. Total of 43 samples (42,39%) were taken from intranodal organs and 56 samples (55,43%) were taken from extranodal organs, while 2 samples (2,17%) were not found. The PCR of the L-Tag genome in 101 samples obtained negative results in all samples. Due to the absence of a positive PCR result, a chi-square test to determine the relationship could not be performed. Conclusion: There was no association between Large T-Antigen Simian Virus 40 genome specific Cytotoxic T Lymphocyte (CTL) epitope I and prevalence of Non-Hodgkin Lymphoma cases in Yogyakarta.

Kata Kunci : Non-Hodgkin's Lymphoma, SV40, L-Tag, CTL epitope I


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