OPTIMASI PRETREATMENT FESES SAPI PERAH PERANAKAN FRIESIAN HOLSTEIN PADA PRODUKSI BIOETANOL GENERASI DUA

ARIYA DWI NUGRAHANTO, Asih Kurniawati, S.Pt., M.Si; Yuny Erwanto, S.Pt., MP., Ph.D

2018 | Skripsi | S1 ILMU DAN INDUSTRI PETERNAKAN

Penelitian ini bertujuan untuk mengetahui pengaruh pretreatment dengan NH4OH 15% dan H2SO4 0,18 N terhadap kandungan Total Bahan Kering (TBK), Bahan Organik (BO), Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), hemiselulosa, selulosa, lignin (ADL), silika dan gula reduksi pada feses sapi perah Peranakan Friesian Holstein (PFH). Perlakuan yang diberikan terdiri dari P0 (feses sapi tanpa perendaman sebagai kontrol), P1 perendaman feses dalam (NH4OH 15% pada 3 jam), P2 (NH4OH 15% pada 4 jam), P3 (NH4OH 15% pada 5 jam) dilanjutkan dengan H2SO4 0,18 N selama 35 menit pada suhu 160°C. Masing-masing perlakuan dilakukan 6 kali pengulangan. Data yang diperoleh dianalisis dengan ananlisis variansi Rancangan Acak Lengkap (RAL) pola searah. Jika terjadi perbedaan karena perlakuan maka dilanjutkan uji Duncans Multiple Range Test (DMRT). Hasil penelitian menunjukan lama perendaman yang berbeda menggunakan NH4OH 15% dan penambahan H2SO4 0,18 N berpengaruh nyata (P<0,05) pada penurunan TBK sesudah pretreatment, kehilangan bahan kering selama pretreatment, NDF, ADF, hemiselulosa, selulosa, silika dan gula reduksi dibanding kontrol. Lama perendaman yang berbeda menggunakan NH4OH 15% dan penambahan H2SO4 0,18 N berpengaruh nyata (P<0,05) pada peningkatan BO dan lignin dibanding kontrol. Berdasarkan hasil penelitian dapat disimpulkan pretreatment yang efektif dalam pembuatan bioetanol yaitu perendaman feses dengan NH4OH 15% selama 5 pada suhu 60°C dan H2SO4 0,18 N selama 35 menit pada suhu 160°C (P3).

The purpose of this study was to determine the effect of pretreatment with NH4OH 15% and H2SO4 0.18 N on total content of dry matter before, after and loss of total content of dry matter (TDM), organic matter (OM), Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), hemicellulose, cellulose, lignin (ADL), silica and reducing sugar in dairy cow feces of Friesian Holstein crossbreed. Treatments consisted of P0 (cow feces without soaking as a control), P1 was soaking feces in (NH4OH 15% for 3 hours), P2 (NH4OH 15% for 4 hours), P3 (NH4OH 15% for 5 hours) at 60°C followed by H2SO4 0.18 N for 35 minutes at a temperature of 160°C. Each treatment had 6 replication. Data obtained was analyzed using analysis of variance Oneway Completely Randomized Design. If there was a difference due to the treatment then continued by Duncan's Multiple Range Test (DMRT) test. The result showed that different soaking time using NH4OH 15% and followed by H2SO4 0.18 N had the significant effect (P<0,05) on the decrease of total dry matter after pretreatment, dry matter loss during pretreatment, NDF, ADF, hemicellulose, cellulose, and reducing sugar compare to control. Different soaking time using NH4OH 15% and the addition of H2SO4 0.18 N had the significant effect (P<0,05) on increasing of OM and lignin. It could be concluded pretreatment that effectively use to make bioethanol was soaking the feces with NH4OH 15% for 5 at 60°C and 0.18 N H2SO4 for 35 min at 160°C (P3).

Kata Kunci : Feses Sapi, Pretreatment, Lignoselulosa, Bioetanol