Latar Belakang. Regenerasi saraf tepi setelah cedera bisa dipacu dengan faktor pertumbuhan dari Platelet Rich Plasma (PRP) yang diaktivasi spons kolagen. Cytidine 5'-diphosphocholine (citicolin) adalah neuroprotektor yang bersifat antioksidan sehingga mampu mengatasi kelemahan dari PRP yang masih mengandung neutrofil dan bisa menghasilkan radikal bebas. Penelitian ini bertujuan untuk mengetahui manfaat PRP yang diaktivasi spons kolagen yang dikombinasikan dengan pemberian cytidine 5'diphosphocholine terhadap proses regenerasi nervus mentalis setelah cedera penjepitan. Metode. Tiga puluh enam ekor tikus Wistar dibagi dalam kelompok Sham Operation dan kelompok tikus yang dicederai nervus mentalisnya (n=6). Kelompok cedera terdiri dari kelompok salin, aplikasi PRP yang diaktivasi spons kolagen. Injeksi citicolin intraperitoneal dengan dosis 50 mg/100gr BB, aplikasi lokal PRP teraktivasi spons kolagen dan injeksi citicolin intraperitoneal 50 mg/100 gr BB, serta aplikasi spons kkolagen. Pemeriksaan histologi regenerasi saraf dilakukan 4 minggu setelah penjepitan nervus mentalis dengan parameter histomorfometri yaitu jumlah serabut saraf, diameter serabut saraf, diameter akson, dan tebal mielin. Hasil penelitian dianalisis dengan anova satu jalur. Hasil. Aplikasi kombinasi Platelet Rich Plasma yang diaktivasi spons kolagen dan cytidine 5'diphosphocholine (citicolin) berbeda bermakna dibandingkan kelompok salin dalam meningkatkan diameter serabut saraf dan tebal mielin. (p<0,05) dan sama dengan kelompok Sham (p>0,05) pada pengukuran jumlah serabut, diameter serabut saraf, dan tebal mielin. Rerata jumlah serabut, diameter serabut, dan tebal mielin pada kelompok PRP-citicolin lebih tinggi dibandingkan kelompok PRP, citicolin, spons kolagen dan salin Kesimpulan. Pemberian aplikasi kombinasi Platelet Rich Plasma yang diaktivasi spons kolagen dan cytidine 5'diphosphocholine mampu meningkatkan proses egenerasi saraf pasca cedera

Background. Regeneration of peripheral nerves after injury could be stimulated by adding growth factors from Platelet Rich Plasma (PRP) which activated with collagen sponge. Cytidine 5'-diphosphocholine (citicolin) is a neuroprotective agent which have antioxidant properties so that could overcome the PRP weakness that still contained neutrophils which could produce free radicals. The aim of this study is to determine the benefits of PRP were activated with collagen sponge combined with the provision of cytidine 5'-diphosphocholine towards the regeneration of mental nerve after crush injury. Methods. Thirty-six Wistar rats were divided into Sham operation and group with mental nerve injury (n=6). The injury group consisted of saline group, local application PRP which activated with collagen sponge, citicoline intraperitoneally 50 mg/100 gr weight, local application collagen spone, and local application of PRP combined with citicoline intraperitoneally 50 mg/100 gr weight. Histological examination of nerve regeneration performed 4 weeks after mental nerve crush injury with histomorfometri parameters were the number of nerve fibers, nerve fiber diameter, axon diameter, and myelin thickness. The result data were analized by one way anova. Result. Application combination of platelet rich plasma which activated with collagen sponge and cytidine 5-diphosphocolin (citicolin) significantly different than the salin group in increasing the number of nerve fibers and thick myelin (p<0,05) and same with Sham group (p> 0.05) in the measurement of the amount of fibers, nerve fiber diameter, the diameter of the axon and myelin thickness. The mean number of fibers, fiber diameter, the diameter of the axon and myelin thickness in the PRP group-citicolin higher than the PRP group, citicolin group, collagen spone group and salin group. Conclusion. Application of a combination of platelet rich plasma activated collagen sponge and cytidine 5-diphosphocolin could improve the process of nerve regeneration after injury.

Kata Kunci : PRP, spons kolagen, citicolin, crush injury nervus mentalis histomorfometri; PRP, collagen sponge, citicolin, crush injury mental nervus, histomorfometry