Latar Belakang: Faktor yang paling bertanggungjawab dalam kesulitan penyembuhan ulkus kronis adalah penuaan fibroblast. Fibroblast tua, yang tidak responsif terhadap rangsangan TGF1, gagal untuk berproliferasi, bermigrasi, dan mensintesis kolagen. Peningkatan kekuatan mikro-mekanis dengan asam hialuronat dapat memperbaiki jalur sinyal TGF1 fibroblast tua manusia. Sebagai limbah rumah pemotongan hewan, vitreous gel mata sapi dianggap sebagai sumber kadar asam hyaluronic tinggi. Tujuan: Untuk mengevaluasi efek dari vitreous gel mata sapi pada fibroblas kulit manusia yang dibuat menua secara artifisial khususnya di tingkat migrasi in vitro. Bahan dan metode: Fibroblas kulit manusia dari enam preputium yang berbeda dirangsang untuk menjadi fibroblas tua melalui kelaparan serum. Setelah itu, mereka diperlakukan dengan berbagai konsentrasi vitreous gel mata sapi yang diencerkan dalam DMEM + 0.2% bovine fetal serum selama 72 jam. Tingkat migrasi sel dianalisis. Data disajikan dalam mean plusminus standar error dan dianalisis dengan ANOVA. Hasil: Kultur sel fibroblas tua pada 50% bovine gel vitreous menunjukkan elevasi signifikan (p <0,05) di tingkat migrasi bila dibandingkan dengan kontrol negatif. Pada konsentrasi 25%, gel vitreous sapi dapat meningkatkan tingkat migrasi secara signifikan (p <0,05) bila dibandingkan dengan kontrol. Tingkat migrasi fibroblast tua di 50% BVG adalah: 46,12plusminus12,37%. Kesimpulan: vitreous gel mata sapi bisa meningkatkan tingkat migrasi fibroblas tua secara signifikan.

Background: The most responsible factor in poor healing of chronic ulcers is wound bed fibroblasts aging. The aged fibroblasts, which are unresponsive to TGF1 stimulation, fail to proliferate, migrate, and synthesize collagen. Increasing the microenvironment mechanical forces with hyaluronic acid may ameliorate TGF1 signaling pathway of aging human fibroblasts. As waste of slaughterhouses, bovine vitreous is considered as a source of high hyaluronic acid level. Objective: To evaluate effects of bovine vitreous gel on artificially aged human dermal fibroblast particularly the migration rate in vitro. Materials and methods: Human dermal fibroblasts from six different human foreskins were stimulated to become senescent fibroblasts by serum starvation. After that, they were treated with various concentrations of bovine vitreous diluted in a DMEM + 0.2% fetal bovine serum for 72 hours. The migration rates of cells were analyzed. Data is presented in mean plusminus standard error and analyzed with ANOVA. Results: Cultivation of senescent fibroblasts in 50% bovine vitreous gel showed significant (p<0,05) elevation in migration rate when compared to negative control. In 25% concentration, bovine vitreous gel could increase migration rate significantly (p<0,05) when compared to control. Migration rate of senescent fibroblasts in 50% BVG was: 46.12plusminus12.37%. Conclusion: Bovine vitreous gel could improve migration rate of senescent fibroblasts significantly.

Kata Kunci : Senescent fibroblasts, bovine vitreous, migration rates, hyaluronic acid, chronic ulcers, mechanical forces